FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346018
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44080.32748447643345036No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35820.26611828370794444No Hit
TCTCAAGACTCCGGGCTGAGGACGAGGCTGATTATTCCTGCAGTTCATAT30500.22659429517287288No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30140.22391973955771766No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGCGTGCTTGTATATGAA28990.21537602023152738No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAAGCCACGAGCGTGCGC27590.20497497061703485No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26820.19925439332906394No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26010.1932366431949647No Hit
GTCTTGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG25090.18640166773401248No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24940.18528726956103114No Hit
GTAATACACGGCCGCGTCCGCGGCGGTCACGGAGGTCAGCTTCAGGGAGAACTGGTTGTTGGACGTGTCTAACGA22900.17013145440848487No Hit
GTGTATTACTGTGCGAAATCGGAAATTGCCCTCCGTACTGGGAGCTACTA22360.16611962098575206No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22100.16418799748591772No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21740.1615134418707625No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19660.1460604538720879No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19530.14509464212217074No Hit
GTTCATATACAAGCACGCGCACGCTCGTGGCTTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG19420.14427741679531775No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19290.14331160504540058No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT19260.1430887254108043No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19060.14160286118016252No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18130.1346935925076782No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17010.12637275281608418No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16570.12310385150867224No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16170.12013212304738867No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16080.11946348414359985No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC15620.11604599641312374No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAATGC15120.11233133583651927No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA14640.10876526168297898No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14600.1084680888368506No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14580.10831950241378643No Hit
GTATATGAACTGCAGGAATAATCAGCCTCGTCCTCAGCCCGGAGTCTTGA14310.10631358570242003No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14190.10542206716403495No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13730.10200457943355884No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13730.10200457943355884No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGTGGTGGTCTCCA13690.1017074065874305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA200.007424626551.6344613
GTATCAA20750.042.4577831
GGTATCA8350.040.8022541
TATCAAC23600.037.0305632
ATCAACG23650.036.952283
TCAACGC23950.036.345754
AACGCAG24400.035.6780936
CAACGCA24500.035.5298235
GTGGTAT3000.034.4140241
AGAGTAC27250.032.08600611
CAGAGTA27300.031.90114410
ACGCAGA27550.031.7307137
CGCAGAG27550.031.6104858
CGCATAT450.00467463830.59706514
GCAGAGT28800.030.239639
TGGTATC3150.029.491162
GAGTACG27450.027.21233412
TCCGTAC6100.026.57298932
CTAACGA6600.025.93079869
GCCGATT15300.025.24014970
Produced by FastQC (version 0.11.5)