FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133285
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10950.039.9886631
GTATCAA24750.038.3093451
ATCAACG28600.032.8923343
TCAACGC28900.032.4316564
TATCAAC28900.032.312422
CAACGCA29600.031.6646945
AACGCAG30000.031.2424956
AGAGTAC32850.028.53196111
CAGAGTA33550.027.93665910
ACGCAGA34100.027.6881717
TCGATAG500.007775261527.57055917
CGCAGAG35100.027.095688
GTGGTAT4550.026.5218561
TGGTATC4450.026.3279462
GCAGAGT37600.025.1108179
AGTACGG28900.023.72757113
GAGTACG29150.023.52407812
GTACTTT11850.021.51847314
GTACGGG32050.021.39553514
TTAACGG2151.382432E-1020.8842935
Produced by FastQC (version 0.11.5)