FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748974
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.042.972191
GTATCAA23550.040.0805361
ATCAACG27850.033.6357883
TCAACGC27850.033.6357884
TATCAAC28000.033.578592
CAACGCA28250.033.4033475
AACGCAG29050.032.4834636
GTGGTAT3200.030.1426831
ACGCAGA31750.029.8295617
CGCAGAG31950.029.7545978
AGAGTAC32350.029.3866911
CAGAGTA32800.028.98351910
GCAGAGT33900.028.1446559
AGTACGG23750.027.99042913
CGGGCTA500.007464966327.80036547
TCGTATG756.0361144E-527.68288240
GTCGTAT500.007640854527.66803439
GAGTACG24200.027.46994612
ACGAGAC1653.6379788E-1227.14146422
GTACGGG26500.025.08576214
Produced by FastQC (version 0.11.5)