FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719846
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT92210.5361526555284601No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC74840.43515524064363903No Hit
GTATGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC64980.3778245261494343No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62760.3649163936771083No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG57520.3344485494631496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG57440.3339833915362189No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57020.3315413124198329No Hit
GGTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGA52490.30520174480738393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49570.2882234804744146No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49240.28630470402582553No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCA49170.28589769083976124No Hit
GTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACACGAACTGTGGCTGCACCAT49070.2853162434310979No Hit
GTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCACTGATTTCACACTGAA46240.26886128176592555No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCCG45340.2636282550879555No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44570.25915111004124786No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT42990.24996424098436718No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42470.2469407144593179No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG42230.24554524067852584No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41380.24060293770488753No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40810.23728868747550652No Hit
GTCTGGGACCCCAGAGTCCCGGTGAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGATGAAA38830.22577602878397252No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38280.2225780680363242No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38210.22217105485025984No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTG38080.2214151732189975No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37980.2208337258103342No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37500.21804277824875018No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35160.2044369088860282No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC34870.20275071140090448No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTGAGCCCCGGAGG33870.19693623731427118No Hit
GTGATGGAAACACCTTCTTGAATTGGTTTCATCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT33610.1954244740517465No Hit
GTCTCCACTCTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCATA32910.19135434219110314No Hit
TTATTACTGCATGCAAGGTTCATACTGGCCTCCGGGGCTCACTTTCGGCG32030.18623760499486583No Hit
GCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTGAGCCCCGGAGGCCAGTATGAACCTTGCATG31600.18373738113761348No Hit
ATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACACGAACTGTGGCTGCACCAT31440.18280706528375215No Hit
GTTTATTACTGCATGCAAGGTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACA30310.17623670956585646No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGT29900.17385277519033682No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29700.17268988037301014No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29020.16873603799409947No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28250.1642588929473918No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28060.16315414287093147No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27230.1583281293790258No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26940.15664193189390213No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA26180.15222293158806077No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25900.15059487884380346No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25220.1466410364648928No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCCG25080.14582701009276414No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24800.1441989573485068No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24730.14379194416244245No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24600.14303606253118012No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTCCACTCTCC24450.1421638914181851No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC24290.14123357556432378No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23860.13873335170707146No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTTCATACTGG23800.13838448326187344No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23590.13716344370368044No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGA23550.1369308647402151No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23340.1357098251820221No Hit
GTATTGACTCAGTCTCCACTCTCCCTGCCCGTAACCCTTGGACAGCCGGC23090.13425620666036378No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23000.13373290399256677No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22780.13245371969350744No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22490.13076752220838378No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22460.13059308798578476No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22460.13059308798578476No Hit
CACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCAC22080.1283835878328641No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTTCATAC22030.12809286412853244No Hit
CGCATACAGTGATGGAAACACCTTCTTGAATTGGTTTCATCAGAGGCCAG21950.12762770620160177No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTCCACT21810.1268136798294731No Hit
CTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA21650.12588336397561176No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21640.12582521923474543No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21430.12460417967655242No Hit
AGGTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGG20840.12117363996543877No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20780.12082477152024076No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20300.11803382395865676No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA20060.11663835017786477No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19820.11524287639707277No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCC19650.1142544158023451No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA19460.11314966572588477No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19400.11280079728068675No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19390.11274265253982042No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19240.11187048142682543No Hit
CCACTCTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAG18990.1104168629051671No Hit
GGCCAGTATGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTC18960.11024242868256809No Hit
CAGTGATGGAAACACCTTCTTGAATTGGTTTCATCAGAGGCCAGGCCAAT18400.10698632319405342No Hit
GGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTCACCGGGACTCTGG18400.10698632319405342No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18330.10657931000798909No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18190.10576528363586042No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18110.10530012570892976No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18060.10500940200459809No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17930.10425352037333575No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTC17910.10413723089160308No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17660.10268361236994475No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT17480.10163700703435075No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17390.10111370436655374No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTCACCGGGAC17270.10041596747615775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10550.044.0271951
GTATCAA28800.042.888581
GACGATA650.001489426438.6344770
CTTATAC3300.038.5769161
CATAACG451.02281294E-438.1982543
TATCAAC33000.037.191212
TCAACGC34900.035.1664734
ATCAACG35100.035.1619873
CAACGCA35150.034.916365
TATACAC4000.034.3784333
GCTATAC601.3757932E-534.378433
AACGCAG36900.033.4467666
TTATACA4000.032.659512
CGCAGAG38750.031.7621578
AGAGTAC39050.031.60802711
CAGAGTA39050.031.51998110
CGCGTAT553.2101586E-431.44234339
CGTGTAT553.2779205E-431.33054431
TATACGT553.302647E-431.29041727
TGGTATC3000.030.940592
Produced by FastQC (version 0.11.5)