FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421618
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA77400.31962101371892676No Hit
GTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGGGCTGGATCCG56670.234017091052346No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT55920.23091998820623233No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT53870.22245457376018843No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAA53230.21981171266483812No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGAATTCACTCTCACCATCAGCAGC52720.21770568272948088No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50580.20886861594190329No Hit
GTGTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGA47830.19751257217282No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC47830.19751257217282No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT45910.18958398888676908No Hit
CTCTAAACTAGACGCCTTATAGATCAGGACCTTGGGGGCTTTCCCTGGCT45140.18640429663142577No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40240.16616989137015004No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG39550.1633205567517255No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGG38020.15700246694565367No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35210.1453986549488813No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA35150.1451508867211922No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG34750.14349909853659826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34180.1411453003735519No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33900.13998904864433614No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33790.1395348068935728No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33410.13796560811820857No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTT33240.13726359813975614No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC33020.1363551146382295No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32580.13453814763517616No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA32040.13230823358597435No Hit
GTAGTAGGTACTCCCACTATAATAGATACTCCCAATCCACTCCAACCCCTTCCCTGGGGGCTGGCGGATCCAGCC31400.12966537249062404No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG31210.1288807731029419No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30840.1273528690321925No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT30520.12603143848451737No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC30040.12404929266300466No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29590.12219103095533648No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT28910.11938299104152679No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT28410.11731825581078437No Hit
GTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCAC28240.11661624583233193No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA28170.11632718290002798No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT28020.11570776233080528No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC27560.11380820591852224No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27040.11166088127855013No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26940.11124793423240163No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26180.10810953668167315No Hit
GATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGAATTCA25430.10501243383555953No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25000.10323676153712105No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24840.10257604626328347No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT24620.1016675627617568No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24470.10104814219253408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6250.045.8136561
TACGGAT23100.042.42339370
TATACCG350.00134886339.4241146
GTATCAA34750.036.0370061
TATCAAC40250.031.1913172
TTGGAGT29950.030.30493570
ATCAACG42000.029.645323
TCAACGC42100.029.411054
CAACGCA42400.029.121615
AACGCAG42850.028.740046
CTACGGA23750.027.8770369
AATAACG658.632212E-426.55300720
AGAGTAC46300.026.463711
ACGTTAG1000.00977229826.30819770
CGCAGAG47750.025.8764028
CAGAGTA47500.025.86780510
TTCGGGG25350.025.4260770
GCAGAGT49750.024.767289
TTAGCGA700.001330980424.65330710
ACGCAGA52150.023.7559037
Produced by FastQC (version 0.11.5)