FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503934
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT21240.4214837657312267No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT6310.12521480987589645RNA PCR Primer, Index 17 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC150.00243202868.440572
CTAATCG200.007594292551.3355189
CTTATAC4450.050.7941
TATACAC4500.050.1897433
TTATACA4950.044.9357262
GTATCAA11050.040.601071
ACTCGAT400.00228909535.3932759
ATCAACG12850.035.152353
TATCAAC12950.034.8809052
CAACGCA13200.034.4795275
TCAACGC13150.034.35044
AACGTCA902.0579134E-433.90471670
CGGCACT450.003738368932.02265566
CGATTGT450.00398757231.6033962
AACGCAG14350.031.477896
TACACAT7150.031.1093485
CCGTCTT5300.030.93878647
GCCGTCT5300.030.89815146
TCGTATG5500.030.84525740
CGTATGC5750.030.73309341
Produced by FastQC (version 0.11.5)