FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503934
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74351.4753916187437244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAT200.00699650352.41095449
CGTTTCG200.00701616252.37378348
TATCGGC200.007203544552.0258942
CGTAAAC651.1779775E-842.28784632
GGACGCT350.001270014639.90383548
GTATCAA10800.039.2650831
ATCAACG11450.036.1364443
CAACGCA11950.034.9140745
TCAACGC12000.034.765154
TATCAAC12050.034.620892
AACGCAG13400.031.3943866
CGCAGAG13650.030.5900868
TACGGCG450.00480181730.4259514
AGAGTAC14000.030.31728611
ACGCAGA13900.030.0399027
GCAGAGT14200.029.8902829
CAGAGTA14600.029.07136710
TATAGAG605.7066395E-428.4960232
GACTAGA500.00669851528.42222862
TATGGAC906.199005E-627.20363250
Produced by FastQC (version 0.11.5)