FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418539
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCGGTTGACACGTCCAGGAACCAGTTCTCCCTGAGG67090.47295139576705325No Hit
GTAGTAGGTACTCCCTTCATAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCC57710.40682702414244515No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47660.3359794831160793No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG46750.3295644321375725No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT45910.32364284661895093No Hit
GTAAAAGACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA45870.3233608663561594No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40430.2850115506165146No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC40420.2849410555508167No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34880.24588678915419315No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG34670.24440639277453774No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32320.22784005233553675No Hit
GTCTTTTACTGTGCGAGATCGTGGTTCGAGTTTCTATATCGTCATGCTTT31000.21853470366341707No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30040.21176717735642095No Hit
GTGCAAGAGAGTGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTC28050.19773865928254353No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27780.19583529250870088No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27090.19097113297554738No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26930.18984321192438136No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA26190.1846265770627385No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT25370.17884598167551263No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC25360.17877548660981477No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC25330.1785640014127211No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24790.1747572678650358No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG24620.1735588517481719No Hit
GTCTCAAGGCACCCTGTCTGTGTCTCCAGGGGAGAGGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCCA24450.17236043563130798No Hit
GTATTACTGTCAACAATATGGTAGCTCACCGCTCACTTTCGGCGGAGGGA24180.1704570688574653No Hit
CATCAGTAGTGGTCGTTACTACTGGGGCTGGATCCGCCAGCCCCCCGGGAAGGGGCTGGAGTGGATTGGGAGTAT23960.16890617741211206No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT23880.168342216886529No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA23260.1639715228132607No Hit
CCATTGCCCCTTGGCCCCAGATATCAAAAGCATGACGATATAGAAACTCGAACCACGATCTCGCACAGTAAAAGA23220.1636895425504692No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGCTAAGTAGTTGTGGCTAACACTCTGA22420.15804993729463906No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA21730.1531857777614856No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21630.15248082710450683No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21310.15022498500217477No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA20880.14719369717716607No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20770.14641825145448945No Hit
CTACTGATGGAGTCACCAGAGACATTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGA20720.14606577612600005No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20710.14599528106030218No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20530.1447263698777404No Hit
CTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCCCCA20490.1444443896149489No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT20410.14388042908936588No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19990.14091963633005508No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG19790.13950973501609754No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTG19690.13880478435911878No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC19570.13795884357074428No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCAATGTCTCTGGTGACTCCATCAGTAGTGGTCGTTA19420.13690141758527613No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19420.13690141758527613No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19350.13640795212539097No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19330.13626696199399524No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG19050.1342931001544547No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCA18940.13351765443177804No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC18910.1333061692346844No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCCACAACTACTTAGCCTGGTACC18770.13231923831491416No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18720.13196676298642476No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG18650.13147329752653963No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG18590.13105032713235237No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT18220.12844200970153094No Hit
GTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAGAGTGAAGCACCTGTGGTTCTT18130.12780755411025005No Hit
GTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC18070.1273845837160628No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG17980.1267501281247819No Hit
GTCATGGATCTCATGTGCAAGAGAGTGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC17960.12660913799338616No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17940.1264681478619904No Hit
CTTATGAAGGGAGTACCTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCC17890.12611567253350103No Hit
ACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCGGTTGACACGTCC17830.12569270213931377No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17730.12498775148233499No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC17490.12329586990558596No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT17460.12308438470849234No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC17450.12301388964279444No Hit
GCCTCAGGGAGAACTGGTTCCTGGACGTGTCAACCGATATGGAGACTCGA17420.12280240444570083No Hit
GTGTATTACTGTCAACAATATGGTAGCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17350.1223089389858157No Hit
CTGTAGTAGGTACTCCCTTCATAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAG17290.12188596859162844No Hit
CTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCGGTTGACACGTCCAGGAACCAGTTCTCCCTGAGG17280.12181547352593054No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17260.1216744833945348No Hit
CCCTGAGGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTCTTTTACTGT17140.12082854260616029No Hit
TTACTACTGGGGCTGGATCCGCCAGCCCCCCGGGAAGGGGCTGGAGTGGA17080.12040557221197302No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAGCTCATC16820.11857270050382825No Hit
CTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCCCCAGTAGTAACGAC16780.11829072024103673No Hit
GGGCTGGAGTGGATTGGGAGTATCTCTTATGAAGGGAGTACCTACTACAG16750.11807923504394309No Hit
ATCTCGCACAGTAAAAGACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGC16720.11786774984684947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16600.11702180905847495No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16590.11695131399277708No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16550.11666933372998557No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA16480.11617586827010044No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC16440.11589388800730893No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG16310.11497745215323654No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16210.11427250149625777No Hit
GTCTCTGGTGACTCCATCAGTAGTGGTCGTTACTACTGGGGCTGGATCCG16030.11300359031369599No Hit
CAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC15860.11180517419683209No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15810.1114526988683427No Hit
GGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCAATGTCTCTGGTGACTCCATCAGTAGTGGT15810.1114526988683427No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG15740.11095923340845758No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC15460.10898537156891702No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15230.10736398505786586No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15200.10715249986077224No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15090.1063770541380956No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCAATGT15010.1058130936125126No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAG14920.1051786380212317No Hit
GGTCAGGCTGACCTGGTTCTTGGTCAGCTCATCCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTG14870.10482616269274231No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC14730.10383923177297205No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14720.10376873670727418No Hit
CATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACA14710.1036982416415763No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA14690.10355725151018054No Hit
GTGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGT14480.10207685513052514No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC14470.10200636006482726No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA14470.10200636006482726No Hit
CACCATAGATGAGGAGCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGCTAAGTAGTTGTGGCTAACACT14440.10179487486773363No Hit
GCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCAATGTC14260.10052596368517186No Hit
GTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGAC14200.1001029932909846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.046.1990431
TCGTATG306.230626E-446.12121640
ATGTGTA1600.038.83109711
TGTATAA1600.038.83109714
GTGTATA1600.038.83109713
GTATCAA18450.037.445331
TGTGTAT1750.035.5027212
TATAAGA1950.033.6326816
AACCGAT17600.033.5772370
TATCAAC21200.032.2362
GTATAAT553.2335264E-431.403021
GTATAAG2000.031.06487815
CGTCAGA2000.031.0637865
ATTAGAG450.004613027430.680283
ATCAACG22250.030.5596283
CAACGCA22650.030.1723275
TCAACGC22550.030.1530694
TGTTAGA5350.029.78687770
GTCAGAT2100.029.5845576
AACGCAG23450.029.2901826
Produced by FastQC (version 0.11.5)