FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418539
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCGGTTGACACGTCCAGGAACCAGTTCTCCCTGAGG60470.42628366227505904No Hit
GTAGTAGGTACTCCCTTCATAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCC58200.41028128236164113No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT51220.3610757265045233No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50830.35832641894230616No Hit
GTAAAAGACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA43860.3091913581508862No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG43360.3056666048659924No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC39010.27500125128741615No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36440.2568840194030619No Hit
GTCTTTTACTGTGCGAGATCGTGGTTCGAGTTTCTATATCGTCATGCTTT31630.22297589280238328No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31610.22283490267098754No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30860.2175477727436468No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29990.21141470202793153No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29300.2065505424947781No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC28230.19900757046510528No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27360.19287449974939005No Hit
GTGCAAGAGAGTGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTC26790.18885628100461108No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT26310.18547251785111302No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25320.17849350634702324No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT24570.1732063764196825No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT24200.17059805898886107No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA24030.16939964287199719No Hit
GTATTACTGTCAACAATATGGTAGCTCACCGCTCACTTTCGGCGGAGGGA23780.16763726622955027No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT23570.16615686984989486No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT23570.16615686984989486No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23430.1651699389301246No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC23390.16488795866733308No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23060.16256162149930314No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22940.16171568071092862No Hit
CATCAGTAGTGGTCGTTACTACTGGGGCTGGATCCGCCAGCCCCCCGGGAAGGGGCTGGAGTGGATTGGGAGTAT22810.16079924485685623No Hit
GTCTCAAGGCACCCTGTCTGTGTCTCCAGGGGAGAGGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCCA22580.15917785834580508No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT22000.15508914453532824No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21760.15339726295857922No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20730.14613627119169792No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA20690.14585429092890645No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGCTTTCTGCTGGTACCAGGCTAAGTAGTTGTGGCTAACACTCTGA20570.14500835014053193No Hit
CCATTGCCCCTTGGCCCCAGATATCAAAAGCATGACGATATAGAAACTCGAACCACGATCTCGCACAGTAAAAGA20490.1444443896149489No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG20440.14409191428645954No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTG20260.14282300310389773No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20250.14275250803819986No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA20160.142118052446919No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT20100.14169508205273174No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT20050.14134260672424234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19690.13880478435911878No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19620.13831131889923365No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA19400.13676042745388037No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA19320.13619646692829734No Hit
CTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCCCCA19320.13619646692829734No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCA19180.1352095360085271No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG19060.13436359522015257No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA18990.13387012976026744No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18800.13253072351200776No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18320.1291469603585097No Hit
GTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC18300.12900597022711396No Hit
GTCATGGATCTCATGTGCAAGAGAGTGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC18270.1287944850300203No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC18240.1285829998329267No Hit
GGGCTGGAGTGGATTGGGAGTATCTCTTATGAAGGGAGTACCTACTACAG18180.12816002943873944No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCAATGTCTCTGGTGACTCCATCAGTAGTGGTCGTTA17940.1264681478619904No Hit
CTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCCCCAGTAGTAACGAC17700.12477626628524137No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC17580.12393032549686686No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG17500.12336636497128382No Hit
GTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAGAGTGAAGCACCTGTGGTTCTT17450.12301388964279444No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17340.12223844392011782No Hit
GTGTATTACTGTCAACAATATGGTAGCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17250.12160398832883693No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCCACAACTACTTAGCCTGGTACC17170.12104002780325392No Hit
CTACTGATGGAGTCACCAGAGACATTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGA17150.12089903767185815No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC17130.1207580475404624No Hit
ACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCGGTTGACACGTCC17120.12068755247476454No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG17070.12033507714627514No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC16930.11934814622650489No Hit
GCCTCAGGGAGAACTGGTTCCTGGACGTGTCAACCGATATGGAGACTCGA16670.11751527451836008No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAGCTCATC16540.11659883866428769No Hit
ATCTCGCACAGTAAAAGACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGC16330.11511844228463228No Hit
CTGTAGTAGGTACTCCCTTCATAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAG16310.11497745215323654No Hit
GGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCAATGTCTCTGGTGACTCCATCAGTAGTGGT16100.11349705577358112No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16090.11342656070788325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15860.11180517419683209No Hit
CTTATGAAGGGAGTACCTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCC15800.11138220380264482No Hit
CTACTACAGTCCGTCCCTCAAGAGTCGAGTCTCCATATCGGTTGACACGTCCAGGAACCAGTTCTCCCTGAGG15660.11039527288287457No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCAATGT15630.11018378768578092No Hit
GTCTCTGGTGACTCCATCAGTAGTGGTCGTTACTACTGGGGCTGGATCCG15620.11011329262008306No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15550.10961982716019793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15380.10842141104333403No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG15270.10764596532065737No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15150.10680002453228286No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15090.1063770541380956No Hit
GGTCAGGCTGACCTGGTTCTTGGTCAGCTCATCCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTG14940.10531962815262745No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT14890.10496715282413809No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC14890.10496715282413809No Hit
TTACTACTGGGGCTGGATCCGCCAGCCCCCCGGGAAGGGGCTGGAGTGGA14850.10468517256134657No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA14790.10426220216715931No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA14630.10313428111599328No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14430.10172437980203576No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14390.10144239953924425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18850.040.6007581
GGTATCA7800.040.219741
TATCAAC22300.033.9983022
ATCAACG23350.032.469473
TCAACGC23600.031.9794884
GCTTATC553.2718043E-431.340061
AACGCAG24300.031.2033846
CAACGCA24350.031.1360195
CGCAGAG26150.028.882418
TAGAGAG605.457994E-428.7182625
AGAGTAC26450.028.29504411
GCAGAGT26950.028.281989
AACTGTG14800.028.0362270
AACCGAT19250.027.91564470
TGTGATT13100.025.44345770
GGGAACG11000.025.35376770
AGTACGG28250.025.02716813
GAGTACG28650.024.79812812
AGCGCTG6550.024.7283975
CAGAGTA30650.024.64279410
Produced by FastQC (version 0.11.5)