FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358340
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC49600.36515158207812476No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCCCTACCACTGGTGTCTGTTGAGTAA30630.2254958257873581No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29560.2176185638352695No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCCCTACCACTGGTGTCTGTTGAGTAACAGTAGTAGT29550.21754494456468926No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23300.17153290045202232No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT22360.16461268901747722No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22250.16380287704109428No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21380.15739800050061103No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG20800.15312808280695556No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG20510.1509931239601278No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC20370.14996245417200404No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19920.14664958699589203No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19850.14613425210183018No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19750.14539805939602748No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAG19570.14407291252558269No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19130.14083366462005092No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19070.14039194899656934No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17440.12839200789198582No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT17040.12544723706877514No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16630.12242884697498417No Hit
GTAGTAGTCAGCTTCATCTTCCACCTGGGCCCCACTGATAGTCAAGGTGG16400.12073560375163803No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15850.11668654386972335No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15240.11219576836432706No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG15230.11212214909374676No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15230.11212214909374676No Hit
GACTACTACTGTTACTCAACAGACACCAGTGGTAGGGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT15110.11123871784678359No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC14790.10888290118821503No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC14710.10829394702357288No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14510.10682156161196754No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14470.10652708452964649No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14380.10586451109442407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14010.1031405980829542No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTC13950.1026988824594726No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13830.10181545121250939No Hit
CAGTAGTAGTCAGCTTCATCTTCCACCTGGGCCCCACTGATAGTCAAGGT13830.10181545121250939No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA13820.10174183194192911No Hit
ATAGTATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA13770.10137373558902778No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA13640.10041668507148431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3800.047.1275671
GTATCAA29750.043.179451
GGTATCA11350.040.0528451
AATTACG350.001315160739.62629344
TGGTATC4500.038.2561152
TATCAAC33950.037.7264982
ATCAACG35200.036.386783
TCAACGC35350.035.9401864
CAACGCA36200.035.0962875
AACGCAG36250.034.9567536
AGAGTAC38300.032.9192211
CGCAGAG38950.032.4571088
CAGAGTA39150.032.0285210
GCAGAGT41400.030.204639
AGTACGG34650.028.3340613
CGCTAAG500.0071151728.07666463
GAGTACG35200.027.89134212
ACGCAGA45850.027.7177687
GTACGGG38700.025.27985614
GAGTACT11050.024.93991512
Produced by FastQC (version 0.11.5)