FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98237
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT16471.6765577124708613No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT4040.41125034355690826TruSeq Adapter, Index 1 (95% over 21bp)
GTATTATTGTCAGGAGTCTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA3870.39394525484287995No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3640.3705324877591946No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC3150.32065311440699534No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2810.2860429369789387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2780.2829890977941102No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT2760.2809532050042245RNA PCR Primer, Index 1 (95% over 23bp)
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2720.2768814194244531No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2710.2758634730295103No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCCTGCAGTCTGAAGATTTTGCAGTGTATTATTGTCAGGAGT2310.23514561723179658No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC2260.23005588525708237No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2010.2046072253835113No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT2000.20358927898856846No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA1960.19951749340879707No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT1930.19646365422396855No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC1920.19544570782902573No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1800.1832303510897116No Hit
CAATAATACACTGCAAAATCTTCAGACTGCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA1800.1832303510897116No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT1790.18221240469476876No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA1790.18221240469476876No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT1790.18221240469476876No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGCGGCCCTGGT1780.18119445829982592No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG1730.17610472632511173No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG1720.17508677993016888No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT1690.17203294074534034No Hit
GAGTTCAGTCTCACCATCAGCGGCCTGCAGTCTGAAGATTTTGCAGTGTATTATTGTCAGGAGTCTACTAACTGG1690.17203294074534034No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG1630.1659252623756833No Hit
CTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT1610.1638893695857976No Hit
CATCTATGGTGCCTCCACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTG1590.16185347679591192No Hit
CTCCTGCAGGGCCAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCT1580.16083553040096907No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG1570.15981758400602625No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT1560.1587996376110834No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCTTCGGAGGCCAGTTAGTAGACTC1520.15472785203131204No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTGAGAGTATTAGCAG1500.15269195924142637No Hit
GTGTATTATTGTCAGGAGTCTACTAACTGGCCTCCGAAGACGTTCGGCCA1490.15167401284648352No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1470.14963812005659782No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA1420.1445483880818836No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1350.1374227633172837No Hit
CTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT1340.13640481692234085No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC1320.1343689241324552No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC1320.1343689241324552No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT1310.13335097773751234No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG1310.13335097773751234No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT1290.13131508494762667No Hit
GTCTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT1260.12826124576279813No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA1240.12622535297291246No Hit
GTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTGAGA1200.12215356739314108No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA1200.12215356739314108No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT1190.12113562099819823No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG1180.12011767460325538No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA1150.11706383541842685No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA1150.11706383541842685No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGATACCAGGC1140.11604588902348403No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG1130.11502794262854119No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT1080.10993821065382696No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC1060.10790231786394129No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC1060.10790231786394129No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC1060.10790231786394129No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG1060.10790231786394129No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG1060.10790231786394129No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT1050.10688437146899844No Hit
GAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCCTC1050.10688437146899844No Hit
CACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGTCTCACCATCAGCGG1020.10383053228416991No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC1010.10281258588922706No Hit
GGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC1010.10281258588922706No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG1000.10179463949428423No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT1000.10179463949428423No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA1000.10179463949428423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAG100.009185701132.84770
GTATAAG150.002541899467.641043
TATAAGA150.002541899467.641044
CTTATAC1700.057.693831
TATACAC1900.053.400823
TTATACA1950.052.0315672
GTCGTAT200.00739435651.65124539
TGTCGTA200.007485776751.49108538
TGTATAA200.00793955350.7307782
GTGTATA200.00793955350.7307781
ATAAGAG200.00793955350.7307785
ATACACA2350.043.1751334
TACACAT2450.041.4128845
CCAGTTG858.827659E-539.07264770
CGGGTGG350.001479625738.65202317
AAATGGG607.207469E-637.71919367
ATCAACG2300.036.7614363
CTGCTTG3000.036.66539454
GCAGAGT2350.035.979289
TCAACGC2350.035.979284
Produced by FastQC (version 0.11.5)