FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98237
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33923.4528741716461213No Hit
GTATTATTGTCAGGAGTCTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA3810.3878375764732229No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3560.36238891659965183No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2820.2870608833738815No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC2680.2728096338446817No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2650.2697557946598532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2450.24939686676099634No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCCTGCAGTCTGAAGATTTTGCAGTGTATTATTGTCAGGAGT2330.23718151002168225No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT2310.23514561723179658No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2170.2208943677025968No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2140.21784052851776828No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC2100.2137687429379969No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA1940.19748160061891143No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG1920.19544570782902573No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT1900.19340981503914004No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA1840.18730213666948298No Hit
CAATAATACACTGCAAAATCTTCAGACTGCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA1830.18628419027454016No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC1750.1781406191149974No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT1740.17712267272005455No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT1700.1730508871402832No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT1680.1710149943503975No Hit
CATCTATGGTGCCTCCACCAGGGCCGCTGGTATCCCAGGCAGGTTCAGTG1660.16897910156051182No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGCGGCCCTGGT1600.1628714231908548No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA1580.16083553040096907No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG1530.15574579842625488No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT1520.15472785203131204No Hit
GTGTATTATTGTCAGGAGTCTACTAACTGGCCTCCGAAGACGTTCGGCCA1480.15065606645154064No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG1480.15065606645154064No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG1480.15065606645154064No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT1470.14963812005659782No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT1430.14556633447682646No Hit
GTCTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT1410.14353044168694076No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCTTCGGAGGCCAGTTAGTAGACTC1400.14251249529199794No Hit
CTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCAT1400.14251249529199794No Hit
CTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGT1390.1414945488970551No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG1390.1414945488970551No Hit
GAGTTCAGTCTCACCATCAGCGGCCTGCAGTCTGAAGATTTTGCAGTGTATTATTGTCAGGAGTCTACTAACTGG1330.13538687052739803No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG1280.13029713855268382No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA1270.12927919215774097No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA1270.12927919215774097No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC1260.12826124576279813No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTGAGAGTATTAGCAG1260.12826124576279813No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG1230.1252074065779696No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC1220.12418946018302676No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT1160.11808178181336972No Hit
CTCCTGCAGGGCCAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGGCTCCCAGGCT1140.11604588902348403No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC1110.11299204983865549No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA1110.11299204983865549No Hit
GTCTGTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTGAGA1090.11095615704876981No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT1080.10993821065382696No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA1060.10790231786394129No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1020.10383053228416991No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC1020.10383053228416991No Hit
GTGCCACCCTCTCCTGCAGGGCCAGTGAGAGTATTAGCAGCAACTTGGCCTGGTATCAACAGAAACCTGGCCAGG1020.10383053228416991No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC1020.10383053228416991No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA1020.10383053228416991No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC1010.10281258588922706No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG1000.10179463949428423No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT990.10077669309934138No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC990.10077669309934138No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCC990.10077669309934138No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC990.10077669309934138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)