FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913641
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACGGCCGTGTCTGAGGCAGTCACAGAGCTCACATTCAGGGAGAACTGGTTCTTGGACGTGTCCACCGA70810.3700276070590043No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55980.2925313577625061No Hit
GTCTCTGGTAGCTCCGTCAGCAGTGGTGATTACTACTGGGGTTGGATCCG51640.2698520777930657No Hit
GTCTACTACTGTGCCAGAGTGGACAACTTTGCCTACGTTTCACACTACAT50610.264469668030733No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA48930.2556905919135303No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT47560.24853146436557327No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT45300.23672151673171715No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGCAGCCTCGAGCCTGAAGATT41600.21738664671168728No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGTCAGTCAGAGTGTGAACAA40690.21263131381486916No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39320.20547218626691213No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT38870.2031206480212328No Hit
CTCCTGCAGGGTCAGTCAGAGTGTGAACAACTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT38500.20118716101922984No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC37190.19434157190403006No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36480.19063136711640272No Hit
GTAGTAGGTGCTCCCACTATAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAACC35740.18676439311239673No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC35200.18394254721758155No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCCCCTGGCCAAAAGTGTACCCCGGAGGCCACTTGTTTCGAA34470.18012782961903512No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34330.17939623994260157No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG34330.17939623994260157No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34140.1784033682388703No Hit
CACCTGCACTGTCTCTGGTAGCTCCGTCAGCAGTGGTGATTACTACTGGG31390.16403285673749674No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31060.1623083953573319No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30960.16178583130273652No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30240.15802337010964962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29630.15483572937661766No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28440.14861721712693238No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27940.1460043968539554No Hit
GTAATAAACTGCGAAATCTTCAGGCTCGAGGCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27120.14171937160627307No Hit
GGCCAAAAGTGTACCCCGGAGGCCACTTGTTTCGAACCTGACAGTAATAAACTGCGAAATCTTCAGGCTCGAGG27070.14145808957897538No Hit
GTTTATTACTGTCAGGTTCGAAACAAGTGGCCTCCGGGGTACACTTTTGGCCAGGGGACCACGCTGGAGATCAAA26910.1406219870916227No Hit
ACCTACTACAGTCCGTCCCTCAAGAGTCGACTCACCATGTCGGTGGACAC26000.13586665419480456No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25140.1313726033252841No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGTCAGTCAGAGTGTGAACAACTTCTTAGCCTGGTA25070.1310068084870673No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC25000.13064101364885056No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24890.1300661931887956No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCGTGGTCCCCTGGCCAAAAGTGTACCCCGGAGG24780.12949137272874064No Hit
CTGCTACTCTGGCTCCCAGATAGCACCGGAGAAAATGTCTTGACACAGTC24150.12619921918478963No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23580.12322060407359582No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA23390.12222773236986458No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23020.12029424536786157No Hit
GACTTCACTCTCATCATCAGCAGCCTCGAGCCTGAAGATTTCGCAGTTTATTACTGTCAGGTTCGAAACAAGTGG22720.11872655320407538No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22610.11815173274402042No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT22440.11726337385120825No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22270.11637501495839606No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA22270.11637501495839606No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT22220.11611373293109836No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT22190.11595696371471975No Hit
GGGTTCCCTGGCCCCAATTGCCAATGTAGTGTGAAACGTAGGCAAAGTTGTCCACTCTGGCACAGTAGTAGACGG21950.11470280998369078No Hit
CTTTTGGCCAGGGGACCACGCTGGAGATCAAACGAACTGTGGCTGCACCA21910.11449378436185262No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21900.11444152795639308No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21890.11438927155093352No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21690.11334414344174273No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21630.1130306050089855No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC21440.11203773330525423No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21420.11193322049433514No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTGTGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAC20850.10895460538314136No Hit
GTCAGAGTGTGAACAACTTCTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTGTATGATG20830.10885009257222227No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20740.1083797849230864No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATACA20690.1081185028957887No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20490.10707337478659791No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19990.10446055451362088No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19650.10268383672799652No Hit
CTCCCAGGCTCCTCGTGTATGATGCATCCAACAGGGCCACTGGCATCCCA19580.10231804188977973No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19470.1017432214297248No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19250.10059358050961492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3850.042.0724071
GGTATCA12050.040.8986781
GTATCAA28400.040.53111
TGGTATC4450.036.394022
TATCAAC32100.035.7463232
ATCAACG33550.034.0986943
TCAACGC33850.033.796494
CAACGCA34550.033.211495
AACGCAG35100.032.7918246
TGTTAGA9000.030.40544770
AGAGTAC37850.030.33730511
CGCAGAG37750.030.3263268
ACCCTGG25300.029.2036570
GCAGAGT39650.029.134019
TTATACC605.4621685E-428.7151414
CAGAGTA41400.027.7359210
TATACCT1254.2091415E-927.5665345
CCACCGA25450.026.3498369
ACGCAGA44000.026.2468577
AGTACGG39950.025.37635413
Produced by FastQC (version 0.11.5)