FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412501
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA42120.29819447915435104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41810.29599979044262625No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT32740.23178744652216174No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28530.2019821578887378No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28070.19872552302617838No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT27910.1975927804652882No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTAGCCCTGGT27090.19178747484072578No Hit
GTATGATAGCCGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT25770.18244234871338144No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25300.1791149174407664No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAG25180.17826536052009875No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT24630.1743715579670386No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCAACTTGGCCTGGTACCAGCAAAAACCTGGCCAGCCTCCCAGGCT24280.17189368361509125No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23940.1694866056731995No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC23850.16884943798269877No Hit
GTAATAGACGGCCGTGTCCTCAGACCCCAGGCTGCTCAGTTCCATGTAGG23380.16552200671008374No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23220.16438926414919353No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA22890.16205298261735743No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22630.1602122759559108No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21730.15384059905090333No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21520.15235387443973492No Hit
TCTCTGGTGCATCCACCAGGGCTACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT21330.15100874264867778No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCAACTTGGCCTGGTA20980.1485308682967304No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19960.14130963447105524No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT19540.1383361852487184No Hit
GTCTATTACTGTGCGAGCGGGCCGTATAAGCTAAAAGTGGGAGTTACTGTTAGAGGGGGGTCCTTATATTACTA18990.13444238269565825No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT18650.13203530475376654No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC18480.13083176578282069No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17930.12693796322976056No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA17870.12651318476942672No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT17760.1257344242588147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17730.12552203502864775No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17580.12446008887781318No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCGGCAG17560.1243184960577019No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17170.12155743606553199No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA17020.12049548991469741No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16990.12028310068453048No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16980.12021230427447485No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15850.11221230993818765No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG15610.11051319609685231No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15540.11001762122646284No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT15390.10895567507562827No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC15350.10867248943540571No Hit
GTTCCATGTAGGCTGTGCTCGTGGATTTGTCCGCGGTAATCGTGAGTCTG14920.10562824380301324No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14900.10548665098290196No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14570.10315036945106588No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC14370.1017344412499531No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG14320.10138045919967491No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14270.1010264771493967No Hit
CACCAGAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTTTGCTGGTACCAGGCCAAGTTGCTGCGAACACTCTGA14260.10095568073934105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.045.3553921
TCGTATG404.9320188E-543.22896240
GTATCAA31300.042.5594941
ACCGATC1300.039.71852527
TATCAAC35850.037.1408422
ATCAACG37400.035.7855723
TCAACGC37450.035.554054
CGATCAG1550.035.54068429
CAACGCA38100.034.9474875
TCCGTAT400.002631176334.40688313
AACGCAG38800.034.316996
ACGCAGA40000.033.3734937
CGCAGAG40700.032.7149668
TATACAC2150.032.005273
AGTATCG553.268429E-431.3455634
GCAGAGT42650.031.2998779
ACGAGAC1700.030.36223622
CCGATCA1850.029.77416628
TTATACA2350.029.2814182
AGTACGG36200.028.32389613
Produced by FastQC (version 0.11.5)