FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412501
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41680.29507943711190293No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37930.2685307833410383No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT28870.2043892358306295No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT27980.19808835533567765No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTAGCCCTGGT26900.19044234304966864No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT26750.18938039689883407No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25780.18251314512343708No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25580.1810972169223243No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24670.17465474360726116No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24410.17281403694581454No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24130.17083173746425664No Hit
GTATGATAGCCGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT23940.1694866056731995No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCAACTTGGCCTGGTACCAGCAAAAACCTGGCCAGCCTCCCAGGCT22850.1617697969771349No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22830.1616282041570236No Hit
GTAATAGACGGCCGTGTCCTCAGACCCCAGGCTGCTCAGTTCCATGTAGG22480.15915032980507623No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA22460.15900873698496495No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC22350.15822997647435294No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAG21930.1552565272520161No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20180.1428671554922793No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCAACTTGGCCTGGTA19620.13890255652916353No Hit
TCTCTGGTGCATCCACCAGGGCTACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT19170.13571671807665978No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19100.1352211432062703No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT18270.12934504117165227No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT18100.12814150220070641No Hit
GTCTATTACTGTGCGAGCGGGCCGTATAAGCTAAAAGTGGGAGTTACTGTTAGAGGGGGGTCCTTATATTACTA18050.1277875201504282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17740.12559283143870342No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC17240.12205301093592147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17150.12141584324542071No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17090.12099106478508687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16880.11950434017391846No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA16880.11950434017391846No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15930.11277868121863277No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15900.11256629198846584No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15890.11249549557841021No Hit
GTTCCATGTAGGCTGTGCTCGTGGATTTGTCCGCGGTAATCGTGAGTCTG15820.11199992070802074No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15660.11086717814713051No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT15430.10923886071585083No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15340.10860169302535007No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15320.10846010020523879No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG15160.10732735764434856No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA14920.10562824380301324No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA14860.10520346534267941No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCGGCAG14470.10244240535050948No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14470.10244240535050948No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC14330.10145125560973053No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14320.10138045919967491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32050.042.224681
GGTATCA12450.039.175741
TATCAAC36500.036.9760932
ATCAACG38150.035.4668853
TCAACGC38350.035.2819184
CAACGCA38850.034.9174735
AACGCAG40200.033.919336
ACGCAGA41600.032.7894257
CGCAGAG42300.032.2536628
GCAGAGT44000.031.0900089
CCTTTGT12250.031.05728770
AGTACGG37550.028.55848113
AGAGTAC51750.026.63326811
GTACGGG42100.024.9821514
CAGAGTA55900.024.71750810
GTACGGA700.001363465624.55061514
GAGTACT12550.024.37455712
AGTACTT12300.024.31109813
TAGACCC851.4892906E-424.2411754
TACGGGA17800.024.1342815
Produced by FastQC (version 0.11.5)