FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551093
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52980.3415655927787696No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGATTCAGGGAGA47750.3078474340352255No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44080.2841866993146123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41880.27000315261560714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38190.24621347656136675No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37940.24460170989102523No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37870.2441504152233296No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA37540.24202288321847884No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT35900.23144969386103864No Hit
GAGTTACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34890.224938156512859No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34860.22474474451241802No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34170.2202962685022755No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC33960.21894238449918862No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33310.21475179115630078No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29070.187416228427309No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28970.1867715217591724No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC28940.18657810975873143No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28870.18612681509103582No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAG28190.18174280974770696No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27700.17858374707383762No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27380.1765206857358005No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27340.17626280306854586No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT27180.1752312723995273No Hit
GTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA26640.17174985639158966No Hit
GTATTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT26410.1702670310548755No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT26400.17020256038806184No Hit
GTTACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA25740.1659474963783603No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25350.16343314037262757No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25320.16323972837218656No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25230.16265949237086363No Hit
GTGTATTACTGTGCGAGGGGCGTTCATAGAAGCAGCTGGACGGGGCGTTTCTTAGTGGGCGGTCCCTACTTTGA25000.16117666703414946No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24160.15576113102180206No Hit
TTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC24130.15556771902136107No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24070.1551808950204791No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23510.15157053767891415No Hit
GTAGTAGGCGAGCCCACTGAAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGCT23380.15073241901033657No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23330.15041006567626827No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23200.1495719470076907No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGATTCAGGGA22820.14712206166877165No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22650.1460260603329394No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA22310.143834057661275No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACCCCCTGGACGTTCGGCCAAGGGA21930.1413841723223559No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21900.14119076032191494No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21240.1369356963122134No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21150.13635546031089046No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20470.13197145496756157No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20340.131133336298984No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20170.13003733496315178No Hit
GGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC20110.12965051096226984No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19890.12823215629236934No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACC19740.12726509629016441No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19490.12565332961982292No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19430.12526650561894098No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT18910.12191403094463066No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18850.12152720694374869No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18790.12114038294286675No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTCCTGTCAACAGAGTTACAATACCCCCTGGACGTT18750.1208825002756121No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT18710.12062461760835747No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18490.11920626293845694No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18460.11901285093801597No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18360.11836814426987936No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA18250.11765896693492911No Hit
GAGTCACCCTCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG18240.11759449626811545No Hit
GTCTCTGGTGGTTCCATCAACAGTGACTACTGGAGCTGGATCCGCCAGCA18210.11740108426767447No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG18080.11656296559909689No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCCTCACTTGCCGGGCAAGTCAGA18010.11611167093140128No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18010.11611167093140128No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG17860.11514461092919637No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGCT17300.11153425358763143No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTG17170.11069613491905385No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16560.1067634242434206No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16540.10663448290979327No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16430.10592530557484302No Hit
ACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA16290.1050227162394518No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT16260.10482930423901082No Hit
GGGTATTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT16210.1045069509049425No Hit
GTCCAGGGGGTATTGTAACTCTGTTGACAGGAGTAAGTTGCAAAATCCTC16090.1037333029031786No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16020.10328200823548297No Hit
ACTTACTCCTGTCAACAGAGTTACAATACCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15770.10167024156514147No Hit
GGAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA15760.10160577089832783No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15710.10128341756425953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)