FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564509
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49390.3156900982992108No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT41060.2624465567152378No Hit
CTACAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG40300.25758880262114187No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38020.2430155403388539No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC35040.2239680308646355No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32640.20862775477801665No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTTTTGGACACAACTATTTGGATTGGTACCTGCAGAAACCAGGGCAGTCTC32590.20830816569287872No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG32540.2079885766077408No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31790.20319474033067245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30030.1919452045338186No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29930.1913060263635428No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29750.1901555056570464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29270.18708745043972264No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC29250.18695961480566747No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA29140.1862565188183641No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28610.18286887451590242No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA28420.18165443599237843No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27960.1787142164091098No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27640.1766688462642273No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25660.16401311849276673No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATGGATCAGGAGTTGTGGAGACTGCCCTGGTTTCTGCAGGTA25620.16375744722465643No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24230.15487287065782301No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24200.15468111720674027No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23490.15014295219778218No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT23210.14835325332101No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22730.1452851981036862No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21970.14042744400959023No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21850.13966043020525928No Hit
CTCCACAACTCCTGATCCATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT21200.13550577209846668No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20890.13352431977061174No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20630.1318624565278947No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACT20430.13058410018734312No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20420.13052018237031554No Hit
ATACTATGTGGAGTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG20330.12994492201706734No Hit
GTTTTGGACACAACTATTTGGATTGGTACCTGCAGAAACCAGGGCAGTCTCCACAACTCCTGATCCATTTGGGTT20230.12930574384679155No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG20070.12828305877435028No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC19990.12777171623812966No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19700.1259180995443299No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT19670.12572634609324715No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19520.12476757883783346No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCC19060.12182735925456487No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18760.11990982474373749No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG18700.11952631784157201No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18670.11933456439048928No Hit
AGTCAGAGCCTCCTGCATAGTTTTGGACACAACTATTTGGATTGGTACCTGCAGAAACCAGGGCAGTCTCCACAA18480.1181201258669653No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18260.11671393389235855No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18120.11581908445397246No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18010.1151159884666691No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17960.1147963993815312No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17950.11473248156450362No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAATTAC17830.11396546776017269No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17710.11319845395584174No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17040.10891596021499396No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGA16910.10808502859363545No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCGTGGACGTTCGGCCAAGGGA16770.10719017915524934No Hit
GTTGTGGAGACTGCCCTGGTTTCTGCAGGTACCAATCCAAATAGTTGTGTCCAAAACTATGCAGGAGGCTCTGA16730.10693450788713903No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA16480.10533656246144957No Hit
CTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC16290.10412212393792557No Hit
GTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT16210.10361078140170493No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16210.10361078140170493No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15890.10156541125682242No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15860.1013736578057397No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15770.10079839745249149No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15720.1004788083673536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.045.058221
TGGTATC2800.044.358972
GTGGTAT3100.040.102051
CGTTTAC654.987505E-737.5793358
GTATCAA20800.036.8566131
TATCAAC24550.030.917772
TCGTATG450.0045568730.75738340
ATCAACG24800.030.745223
TCAACGC25000.030.4992584
CAACGCA25800.029.6872715
AACGCAG26150.029.2899276
TGTTAGA7650.028.64818270
CGCAGAG28250.027.1126218
AGAGTAC28050.027.06166611
TTCGGGG13200.026.85767270
TACACAA11800.026.76662870
CAGAGTA28750.026.40277510
AGGTACG809.561317E-525.8760662
CGTATGC700.001308847824.72367941
ACGCAGA31400.024.3927257
Produced by FastQC (version 0.11.5)