FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168339
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCCAATGGTCACCA26300.22510589820249086No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCCA24030.2056766058481314No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22190.1899277521335845No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC20060.171696742127071No Hit
AGCCAGGACAGGCCCCGATACTTGTCTTCTATGGTAAGAACAACCGGCCC19420.16621887996548945No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16590.14199645821974616No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16110.13788806159856No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15680.1342076229587474No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14410.12333749023185907No Hit
CCACTGGTGTCTCGGGAGTTACAGAAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATAGTCAAGGAAG13930.11922909361067294No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTACTGTGTCTGTGGCCTTGGGA13480.11537747177831092No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13270.11358004825654198No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12550.10741745332476277No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12550.10741745332476277No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12510.10707508693966392No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC12400.10613357938064208No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12110.10365142308867548No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11960.10236754914455479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20900.042.0674321
GTGGTAT2450.039.4043461
GGTATCA8750.038.2222141
TATCAAC24950.035.3528182
TCAACGC25400.034.997794
CAACGCA25550.034.7923245
TCGTATG806.920891E-834.59733640
ACTAGCG807.148992E-834.46994427
ATCAACG26000.034.0576323
AACGCAG26650.033.3562476
CTAGCGC851.2159035E-732.44785728
CGTATGC751.6258291E-632.30889541
AGAGTAC28250.031.22712111
CGCAGAG28700.030.8562458
CAGAGTA28950.030.82915510
ACGCAGA28850.030.8126167
TAGCGCT902.0075277E-730.64519929
AATCGAG1406.0121136E-527.69802370
TGGTATC3900.027.3874592
AGTACGG24950.027.34670613
Produced by FastQC (version 0.11.5)