FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168339
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCCAATGGTCACCA22730.19454969833241895No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGCCA21160.18111181771728924No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20540.17580513874825715No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC20020.17135437574197215No Hit
AGCCAGGACAGGCCCCGATACTTGTCTTCTATGGTAAGAACAACCGGCCC19500.16690361273568716No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15980.13677537084698876No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14160.12119770032499129No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTACTGTGTCTGTGGCCTTGGGA13820.1182875860516511No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13780.11794521966655225No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13420.11486392220066266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12800.10955724323163055No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12720.10887251046143286No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12490.1069039037471145No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12320.1054488466104444No Hit
CCACTGGTGTCTCGGGAGTTACAGAAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATAGTCAAGGAAG12300.10527766341789499No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12240.1047641138402467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4100.041.122131
GTATCAA23850.040.9725881
TGGTATC4300.039.204442
GGTATCA9800.035.8127371
TATCAAC27500.035.5298462
ATCAACG28950.033.7502823
TCAACGC28950.033.7502824
CAACGCA29750.032.8427125
AACGCAG30000.032.8025936
CGCAGAG32150.030.4131328
AGAGTAC32150.030.09573211
ACGCAGA32600.030.095067
CAGAGTA32750.029.8597810
AGTACGG29950.026.67292813
GAGTACG30150.026.610212
AACGAAC2051.8189894E-1223.75972651
GTACGGG33500.023.53803614
GTATTAG2053.6379788E-1223.4983621
AATGGTA4500.023.31548570
TACGGGA12900.023.22051615
Produced by FastQC (version 0.11.5)