FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866966
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT80710.432305676696844No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAATTTACTACTGTCAGCAACG53740.2878466988686457No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT50380.26984958483443194No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49880.26717144286505484No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACGTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT48920.2620294102838509No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA47940.2567802520238719No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT47510.25447704993020764No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45730.24494286451922528No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44040.23589074466273088No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41390.22169659222503246No Hit
GCCTAGAGCCTGAAGATTTTGCAATTTACTACTGTCAGCAACGCAGCGACTGGCCGGCAACTTTTGGCCAGGGGA40180.21521548865914No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG40030.21441204606832692No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGGT39520.2116803412595623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38100.20407441806653148No Hit
GGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACT36980.19807538005512684No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35930.19245128191943506No Hit
GAGTATTAGCAGCTACGTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC34780.18629155538986783No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33020.1768644956576606No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31940.17107970900380617No Hit
GCCCTGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTG31230.16727674740729076No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30010.16074208100201073No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAATTTACTACTGTCAGCAACGCAGCGACTGG29790.15956369853548483No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC29180.15629636533284483No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACGTAGCCTGGTA27830.1490653820155268No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27590.1477798738702258No Hit
TCTATGATGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT27290.14617298868859957No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27150.145423108937174No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27060.14494104338268612No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26030.1394240709257694No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25730.13781718574414317No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25530.13674592895639234No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24800.13283584168110185No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA23920.12812231181499822No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTGTGTTGACACAGTC23780.12737243206357268No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23620.126515426633372No Hit
CATCTATGATGCATCCACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23610.12646186379398447No Hit
CTGCAGGAGAGCGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG23510.12592623540010905No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22980.12308740491256938No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22920.12276602787624413No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22720.1216947710884933No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22720.1216947710884933No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22260.11923088047666641No Hit
GAGTACGGGAGAGCCCTGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT22110.1184274378858533No Hit
CACCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG21250.11382103369852478No Hit
GGCCAAAAGTTGCCGGCCAGTCGCTGCGTTGCTGACAGTAGTAAATTGCA21240.11376747085913724No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20960.11226771135628608No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20960.11226771135628608No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAATTTA20910.11199989715934837No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20710.11092864037159755No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20690.11082151469282246No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20380.1091610666718087No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATCCCA20310.10878612679609591No Hit
CACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAATTTACTACTGTCAGC19550.10471535100264279No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19150.10257283742714114No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGC19080.10219789755142837No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC19070.10214433471204083No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCACCAGGGCCACTGGCATC18960.10155514347877787No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18740.10037676101225196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7850.055.1488341
TGGTATC9150.046.9052772
GGTATCA24400.042.6666261
GTATCAA57250.037.869481
ATAAACG959.256837E-932.516253
TATCAAC67500.032.1982842
ATCAACG69800.031.2248123
TCAACGC69900.031.1784734
AACCGTA450.00428865631.14139461
CGCAGAG76100.028.8707668
AGAGTAC76000.028.5466311
GCAGAGT77900.028.2036619
ACGCAGA79450.027.739857
GTCTAAC500.00789272527.4868811
GCTGACA34500.025.83734170
AGCGCTG7050.025.3173985
CAGCGCT7200.024.3106144
GAGTACG66300.024.28216412
AGTACGG66050.024.2181613
CGCGGTA1652.9758667E-923.11086543
Produced by FastQC (version 0.11.5)