FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798298
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38400.4810233772350676No Hit
GTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTT35370.4430676263751131No Hit
GTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA31970.40047701484909143No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31500.3945894891381414No Hit
ATCCATAACTGTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC25410.3183021879047674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24820.31091146413995774No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24300.3043976059065662No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24110.3020175423212885No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23840.2986353467001045No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23750.2975079481597098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22890.2867350287737161No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21820.2733315127934681No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA21740.2723293807575617No Hit
CTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA21410.2681955861094478No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21200.2655649895151936No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20000.25053300897659775No Hit
GGTCTAGTCAGAGCCTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC19790.24790241238234345No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT19660.24627394782399556No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19170.24013588910406888No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAAAAATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18860.23625262746493164No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18830.23587682795146672No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG18820.23575156144697845No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18750.23487469591556034No Hit
GAGTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT18380.23023983524949326No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18290.2291124367090986No Hit
CTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG17920.22447757604303156No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17580.2202185148904294No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17250.2160847202423155No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG16660.2086939964775059No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16360.20493600134285692No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15750.1972947445690707No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15540.19466414797481643No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15470.19378728244339832No Hit
GTCTCCACTCTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCA15370.19253461739851535No Hit
CTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCC15290.19153248536260895No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCGTTCACTTTTGGCCAGGGGA15080.18890188876835468No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15040.18840082275040146No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14960.1873986907144951No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCC14900.1866470916875653No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14820.1856449596516589No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14500.18163643150803332No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14170.1775026368599195No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13890.1739951747342471No Hit
GGCCAAAAGTGAACGGAGTTTGTAGACCTTGCATGCAGTAATAAACCCCA13680.17136457813999284No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13580.17011191309510987No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12870.16121799127644063No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGACCGTCGCCC12840.16084219176297573No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12560.15733472963730336No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12390.15520519906100227No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12260.15357673450265438No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12050.15094613790840014No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT11950.14969347286351714No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA11890.14894187383658733No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11800.14781447529619265No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATAACTGTGC11710.14668707675579795No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11640.14581021122437987No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11620.14555967821540328No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11440.14330488113461387No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC11210.14042375153138303No Hit
GTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCC11070.13867002046854682No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACT11010.13791842144161703No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10960.13729208891917555No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10910.13666575639673406No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10870.13616469037878084No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG10630.13315829427106168No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10630.13315829427106168No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCA10520.1317803627216904No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10320.1292750326319244No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC10250.12839816710050633No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA10040.12576757050625206No Hit
CTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCCCT9880.12376330643443928No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9750.12213484187609139No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9740.12200957537160308No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9680.12125797634467331No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9670.121132709840185No Hit
AGTCAGAGCCTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG9500.11900317926388392No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9490.1188779127593956No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9410.11787578072348923No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9360.11724944820104774No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9230.11562098364269985No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9080.11374198607537536No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT8990.11261458753498069No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8900.11148718899458597No Hit
GTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGCGACGGTCAGGGAGAGTGGAGA8640.10823025987789021No Hit
GTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTA8480.10622599580607742No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8450.10585019629261253No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8380.10497333076119443No Hit
CCACTCTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAG8380.10497333076119443No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8350.10459753124772955No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8260.10347013270733485No Hit
CCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAAAAATCAGGAGCTGTGGAGACTGCCCTGG8170.10234273416694017No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8140.10196693465347527No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8100.10146586863552208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)