FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493099
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147050.9848643659931458No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC18320.12269782512746978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16600.044.8223461
GTATCAA108350.038.6857381
TTACGTC558.652831E-636.78488524
GGTATCA49050.033.839061
TATCAAC123650.033.81952
TGGTATC21900.033.335412
ATCAACG132900.031.4266223
TCAACGC132750.031.2869034
GCGTTAT850.004545709630.77083670
CAACGCA135350.030.7676535
AACGCAG138150.030.297266
ACGCAGA148250.028.1906057
CGCAGAG152050.027.486078
AGAGTAC153500.027.33603911
CAGAGTA156600.026.8378810
GCAGAGT156650.026.807839
TCCCGTT951.1032886E-525.26247236
GTTTACG1001.3214636E-524.68112247
TTACGTA1151.3586123E-624.6229349
AGTACGG103100.024.60301613
Produced by FastQC (version 0.11.5)