FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267834
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT8020.299439204880635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.29271862422246614No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC7800.29122516185398417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.22775301119350044No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC5050.18854962402084874No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.16316076375665525No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT3720.13889200026882323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.1336648819791363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.12321064539976255No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG3090.1153699679652322No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2970.11088958085978629No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.10752929053070186No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT2720.10155544105677397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCTG451.8189894E-1275.5614750
CGTGTTA205.78797E-574.56505648
GTTTCCG150.002063951571.3203743
TGTAGTT150.00247528268.1267138
GTGTTCT452.055458E-1066.73472649
AGGGGGG7700.065.99879570
CGTGTAA304.212434E-665.47610557
AGCTAGT150.003296010463.3796041
TATTCCG352.60643E-761.1317543
TGTTATG251.6357479E-460.44917750
GTCGTCT200.0052647356.30742349
CTTGTAA351.0507261E-556.1223857
GCTTTAA351.0740143E-555.9151956
CCGTGTT800.055.4968947
CGCGTTT750.055.0876439
CGCAGAC250.006219653453.98106468
TTTACGG200.00623333653.9553144
TTTCCGG200.00623333653.9553144
GTAGTTT200.00741167951.6446639
ATGGTTG458.3569466E-751.6394254
Produced by FastQC (version 0.11.5)