FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267834
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138505.1711134508688215No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC9120.3405094200138892No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC6020.22476608645653653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.18294914013904134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.1661476884936192No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC4280.1598004734275708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.14673267770335358No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG3570.13329151638701583No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3430.12806439809732895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.12097045184703958No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.1149966023731117No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG3030.11312977441250924No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC2970.11088958085978629No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2940.10976948408342481No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG2700.100808709872533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.1004353442804125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.1004353442804125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTT300.001881092172.9843570
TATAGTT150.002133658470.7258542
ATACGAA150.002197651470.2009941
GATACGA150.002300026869.3998440
AGCGATA150.002589746467.3546337
GATATGA150.00318061763.95211422
TGTTTCG150.003197221663.86815618
TTGAGTA150.003247423363.61763
TACCTTG150.003247423363.617613
TGAGTAC150.003254642563.5819634
CGATCCC200.003480655162.50826366
CATACGG200.004011405660.30903663
CCATACG200.004128996359.8707862
TTTACCA250.00624469353.92636568
GTGGTAT5350.052.3503531
CCAGGTA300.00833645150.12437469
GTTAGGT450.00936825948.65623570
ATATGAA200.00989824148.00014523
AAATTGA200.00989824148.00014523
TGGGATA200.00997194647.91010319
Produced by FastQC (version 0.11.5)