FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394505
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42670.3059867121308278No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28730.206022925697649No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27860.199784152799739No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG26730.1916809190357869No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26270.1883822575035586No Hit
CTTTTGTACAGGTCCAGCAATAAGAACAACTTAGCCTGGTACCAGCAGAA25800.185011885938021No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25740.18458162573816514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23100.1656501769445072No Hit
CTCCTCAGTTGCTCATTTATTGGGCATCTATTCGGGAATCCGGGGTCCCT21830.15654300271422478No Hit
TTTCAGGGGCAGCGGGTCCGGGACAGATTTCACTCTCACCATCAGCGGCC21400.15345947128192441No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20890.14980225958314958No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20720.1485831890168913No Hit
CTATGAAACTGCTGACAGGAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTC19960.1431332264853837No Hit
GTTTCATAGTCTTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19790.14191415591912543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19090.13689445358747368No Hit
GTCCCTGACCGTTTCAGGGGCAGCGGGTCCGGGACAGATTTCACTCTCAC18890.1354602529212875No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18860.13524512282135956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17520.12563597835791193No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17220.12348467735863262No Hit
GTTTATTCCTGTCAGCAGTTTCATAGTCTTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA16730.11997088572647642No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16520.1184649750269809No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTCCTGTCAGCAGTTTCATAGTCTT16440.11789129476050642No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTGTA16390.11753274459395988No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16260.11660051416093882No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16250.1165288041276295No Hit
CACCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTCCTGTCAGCAGTTTCATAGTCTTCCACTCACTTT15750.112943302462164No Hit
ACTGAGGAGGGTGTCCTGGTTTCTGCTGGTACCAGGCTAAGTTGTTCTTATTGCTGGACCTGTACAAAAGACT15310.10978806099655435No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14940.10713478976410984No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14810.10620255933108881No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14730.10562887906461432No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14430.103477578065335No Hit
TCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTCCCGGACCCGCTGCCCCTGAAACGGTCAGGGACC14300.10254534763231397No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14270.10233021753238605No Hit
GGAATAAACTGCCACATCTTCAGCCTGCAGGCCGCTGATGGTGAGAGTGAAATCTGTCCCGGACCCGCTGCCC14200.10182824729922087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)