FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162256
Sequences flagged as poor quality0
Sequence length20-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATTAAACTATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCT11660.7186174933438516No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC5840.3599250567005226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.30507346415540876No Hit
ATTCTTAGGTAGAATCCATAACCCTTCTTATAAAAGTTTACATTCCGTTGCCTAACTTTTATAAGGTTGCTAGTT4320.2662459323538113No Hit
ATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCTTAGGTAGAATCCATAACCCTTCTTATAAAAGTTTA3760.2317325707523913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.2175574400946652No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2620.16147322749235776No Hit
GTTTTAGATTAATGAGGTTTCAATTAAACTATGCAAAAGATTCGCTCGGG2600.1602406074351642No Hit
AATCAATCCAGTCCAGTGGTTTCTTCTTCCTTTCCATTTTTGCGTCCCAGTCAGATGTTTTTGAGACCCAATTGG2540.15654274726358347No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT2540.15654274726358347No Hit
ATCCATAACCCTTCTTATAAAAGTTTACATTCCGTTGCCTAACTTTTATA2470.15222857706340598No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.13620451631988956Clontech SMART CDS Primer II A (100% over 21bp)
GGGCAAAAATTCGCGCGATTCTTAGGTAGAATCCATAACCCTTCTTATAA2120.1306577260625185No Hit
ATCCAGTCCAGTGGTTTCTTCTTCCTTTCCATTTTTGCGTCCCAGTCAGA1920.11833152549058279No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1870.11524997534759886Clontech Universal Primer Mix Long (95% over 23bp)
GTCATAATCAATCCAGTCCAGTGGTTTCTTCTTCCTTTCCATTTTTGCGT1850.11401735529040528No Hit
ACCAACGACTGACCAATGCACATGGAAATGGACACGGAAACAGTTCGACG1830.11278473523321172No Hit
CCATAACCCTTCTTATAAAAGTTTACATTCCGTTGCCTAACTTTTATAAGGTTGCTAGTTCAAAACGAGCCAGAA1800.11093580514742135No Hit
AGATTAATGAGGTTTCAATTAAACTATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCTTAGGTAGAAT1790.11031949511882458No Hit
AGATGGGACAATGCGTAACGTGACTTTAAACCGAGGCCCCAGTAGGATGAAGAAAGTTTTGAATAACATCTCAAA1740.10723794497584066No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1710.10538901489005029Clontech Universal Primer Mix Long (95% over 22bp)
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1660.10230746474706635No Hit
GTTCTGGCTCGTTTTGAACTAGCAACCTTATAAAAGTTAGGCAACGGAAT1660.10230746474706635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.10230746474706635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.10230746474706635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)