FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162256
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14120.8702297603786608No Hit
CAATTAAACTATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCT8800.5423528251651711No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC5550.34205206587121584No Hit
ATTCTTAGGTAGAATCCATAACCCTTCTTATAAAAGTTTACATTCCGTTGCCTAACTTTTATAAGGTTGCTAGTT4160.2563849718962627No Hit
ATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCTTAGGTAGAATCCATAACCCTTCTTATAAAAGTTTA3100.19105610886500343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.1879745587220195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.16948525786411597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.1509959570062124No Hit
ATCCATAACCCTTCTTATAAAAGTTTACATTCCGTTGCCTAACTTTTATA2360.14544916674884134No Hit
GTTTTAGATTAATGAGGTTTCAATTAAACTATGCAAAAGATTCGCTCGGG2340.14421654669164777No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2290.14113499654866385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.14113499654866385No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG2180.1343555862340992Clontech Universal Primer Mix Long (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.12880879597672812No Hit
AATCAATCCAGTCCAGTGGTTTCTTCTTCCTTTCCATTTTTGCGTCCCAGTCAGATGTTTTTGAGACCCAATTGG2090.12880879597672812No Hit
ACCAACGACTGACCAATGCACATGGAAATGGACACGGAAACAGTTCGACG1960.12079676560496992No Hit
GGGCAAAAATTCGCGCGATTCTTAGGTAGAATCCATAACCCTTCTTATAA1940.11956414554777635No Hit
ATCCAGTCCAGTGGTTTCTTCTTCCTTTCCATTTTTGCGTCCCAGTCAGA1940.11956414554777635No Hit
CCTGAGGTTCTGGCTCGTTTTGAACTAGCAACCTTATAAAAGTTAGGCAA1840.1134010452618085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.11216842520461494No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1780.10970318509022779Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1770.109086875061631No Hit
GTTCTGGCTCGTTTTGAACTAGCAACCTTATAAAAGTTAGGCAACGGAAT1750.10785425500443742No Hit
GAAATGGACACGGAAACAGTTCGACGGACGCCTGAGGTTCTGGCTCGTTT1730.10662163494724386No Hit
AGATTAATGAGGTTTCAATTAAACTATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCTTAGGTAGAAT1710.10538901489005029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1670.10292377477566314No Hit
CCATAACCCTTCTTATAAAAGTTTACATTCCGTTGCCTAACTTTTATAAGGTTGCTAGTTCAAAACGAGCCAGAA1630.10045853466127601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)