FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308220
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31670.24208466465884945No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT30640.23421137117610188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19250.14714650441057314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGT200.00802311950.6316519
TGTCGTA709.094947E-1249.19857438
CTTATAC10800.044.0868261
TCGTATG8100.043.58692640
AGGGGGG10750.042.16158770
GTGGTAT12300.041.7303431
CTCGTAT7300.040.6655539
GTATCAA101000.039.2852941
TATACAC12550.039.2696083
TCTCGTA7300.038.2131438
CGTATGC10100.038.13436541
ATCTCGT7550.037.32243737
GGTATCA44800.037.0851971
GCCGTCT9150.036.88363346
TATGCCG10900.035.84399843
ACGAGAC12850.035.742322
ATGCCGT10500.035.29839744
TTATACA14250.034.347932
TATCAAC119100.034.0108072
TGCCGTC10500.033.71615245
Produced by FastQC (version 0.11.5)