FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308220
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107880.8246319426396173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13950.040.5258871
GGTATCA48000.039.8904081
GTATCAA112000.038.578421
TATCAAC124950.034.501152
CGACTGA450.00337001532.71214365
TCAACGC135350.031.7459244
CAACGCA136650.031.4475215
ATCAACG137200.031.3644773
TGGTATC18450.030.8015172
AACGCAG140050.030.7596266
ACGCATA450.005238638729.8925177
CGCAGAG149950.028.8409468
ACGCAGA149850.028.8377517
CCGTATC905.3332424E-627.74083952
CAGAGTA157200.027.53220610
GCAGAGT156700.027.5127539
AGAGTAC157600.027.26924111
AGTACGG92100.025.59020613
CGTATCG700.001082001625.54362153
GAGTACG95100.025.10496712
Produced by FastQC (version 0.11.5)