FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700579
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37980.22333569919421561No Hit
GTGTGTACCTTGCATGCAGTAATAAATTCCAACATCGTCAGCCTCCACCC31070.18270247956725327No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA30850.18140880253137312No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27910.1641205730518841No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25840.15194824821428465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25810.15177183770939193No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA24430.14365695448432564No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23950.1408343864060417No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23880.1404227618946253No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGACCTGGCTTTTTCTTGTGCCTATTTTAAAAGGTGTC23870.14036395839299437No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23360.1373649798098177No Hit
GGTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22280.13101420163367888No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21950.1290736860798587No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21820.12830924055865678No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21600.12701556352277665No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21370.1256630829852656No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20570.1209588028547924No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC20320.11948871531401951No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20160.11854785928792487No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19960.11737178925530656No Hit
CTCGTATACAGTGATGGAAACATCTACTTGAATTGGTTTCATCAGAGGCC19670.11566648770801004No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTCATTTCACACTGAA19620.11537247019985546No Hit
CTCCTGTGTAGCCTCTGGATTCAGGTTTAGCAGTTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG18960.11149143909221507No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18790.1104917795644895No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18530.10896288852208572No Hit
CGTATACAGTGATGGAAACATCTACTTGAATTGGTTTCATCAGAGGCCAG17820.10478783990629074No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17070.10037757728397209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.041.9223981
GTATCAA46500.038.4025961
TATCAAC54150.032.8991172
ATCAACG55700.032.107343
TCAACGC56450.031.6197174
CAACGCA57600.031.0482465
AACGCAG58850.030.3302146
CGCAGAG63250.028.1063698
TTCGGGG16600.027.78280370
AGAGTAC64700.027.42403211
CAGAGTA65350.027.20398110
GCAGAGT66600.026.692619
CGAGACT2500.026.19128823
ACGAACC1900.025.64361252
CGAACCT1801.0913936E-1125.14526453
TAACGGC1801.2732926E-1124.9480836
TGTTAGA8150.024.80582470
TTAATCG2001.8189894E-1224.18686537
GAGTACG45150.023.9607412
AGTACGG45650.023.69830113
Produced by FastQC (version 0.11.5)