FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700579
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAATTCCAACATCGTCAGCCTCCACCC37050.21786697354254053No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33340.196050874437471No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33010.1941103588836508No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA24070.1415400284256127No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGACCTGGCTTTTTCTTGTGCCTATTTTAAAAGGTGTC23010.1353068572527357No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22990.13518925024947384No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22720.13360155570543913No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21810.12825043705702588No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20130.11837144878303213No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19970.11743059275693749No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19860.11678375423899742No Hit
GGTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19760.11619571922268827No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19510.1147256316819154No Hit
CTCCTGTGTAGCCTCTGGATTCAGGTTTAGCAGTTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG19260.11325554414114251No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18840.11078579707264409No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA18600.10937451303350211No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18490.10872767451556205No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18360.10796322899436016No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC17630.10367057337530336No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17550.10320014536225604No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17430.10249450334268505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)