FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254231
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC34920.2784176120666767No Hit
GACTTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCCACCCCACACGG34500.27506894662944864No Hit
CCCCTGTACTTGTCATCTATGGTAAAAACATCCGGCCCTCTGGGATCCCA31310.24963503533240688No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29690.23671875436024145No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28680.22866601128500252No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACATCCGGCCC27270.21742406303145115No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26000.20729833659030913No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGGTTGCATAATAGTTT25150.20052127558639518No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24600.19613611846621554No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTC24580.19597665820729993No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24540.1956577376894687No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24370.1943023254886859No Hit
GTACAGTAATAGTCAGCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAG24010.1914320408282047No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22690.1809076637397736No Hit
GCCTTGGGCTGACTTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCCACCCCACACGGATATCACTGCTGTCC22590.18011036244519552No Hit
TTACTGTACCTCCCGGGACAGCAGTGATATCCGTGTGGGGTGGATATTCG22370.17835629959712365No Hit
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA22010.17548601493664245No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21420.1707819372986316No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21290.16974544561568003No Hit
GACTATTACTGTACCTCCCGGGACAGCAGTGATATCCGTGTGGGGTGGAT21070.16799138276760822No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA20720.1652008282365848No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20620.16440352694200672No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20510.16352649551797077No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19660.15674943451405682No Hit
GTAATAGTCAGCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG19420.15483591140706934No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19170.15284265817062406No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAA18960.15116832545201003No Hit
GAATAAATACTATGCAGATTCCGTGAGGGGCCGATTCACCCTCTCCAGAGACAATTCCAAGAACACGCTGTAT18650.1486966914388179No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17980.14335477276514455No Hit
GGATGAGGCTGACTATTACTGTACCTCCCGGGACAGCAGTGATATCCGTGTGGGGTGGATATTCGGCGGAGGGAC17720.14128178939924144No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA17710.14120205926978363No Hit
GTTTATTACTGTGCGAAAGTTGAGTGTGGTGGTGACTGCTATACTAATACCTTTGACTACTGGGGCCAGGGAACC17610.14040475797520552No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT17560.14000610732791646No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17330.13817231435038682No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17120.13649798163177276No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17010.13562095020773685No Hit
CTATTATGCAACCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACATCCGGCC16740.13346823671237595No Hit
GTCCTGGCTTCTGCTGGTACCAGGTTGCATAATAGTTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT16550.13195336425267754No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16340.1302790315340635No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16280.12980065075731664No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15900.1267709058379198No Hit
ACCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATCTATGGTAAAAACATCCGGCCCTCTGGGATC15780.12581414428442608No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15750.12557495389605264No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAACTATTATGCAACCTGGTA15750.12557495389605264No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15580.12421954169526984No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15540.1239006211774386No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15400.12278439936502925No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15190.12111006664641522No Hit
GTATTTATTCCTTCCATCAAATGTTATAAATGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG15140.12071141599912616No Hit
ATCCCAGACCGAGTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGAC14940.11911681340996992No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14800.11800059159756057No Hit
GCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAGCCAGAGACTCGGT14620.11656544926731997No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14420.11497084667816376No Hit
CTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTTTATTA14130.11265867292388723No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGGTAC13890.11074514981689976No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13860.11050595942852631No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13660.10891135683937012No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCT13600.10843297606262323No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13550.10803432541533417No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13540.10795459528587636No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13370.10659918308509357No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13280.10588161191997328No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG13250.10564242153159983No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13200.10524377088431079No Hit
GTCTGGGATCCCAGAGGGCCGGATGTTTTTACCATAGATGACAAGTACAGGGGCCTGTCCTGGCTTCTGCTGGTA13060.10412754907190142No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13030.103888358683528No Hit
TCACTGGGGCTCAGGCGGAGGATGAGGCTGACTATTACTGTACCTCCCGG12700.10125726441142023No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG12680.1010978041525046No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12620.10061942337575773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)