FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42470
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT11412.6866023075111842No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT2200.5180127148575465TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATGTCGTATGCCGT1560.36731810689898753No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT1140.2684247704261832RNA PCR Primer, Index 7 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCAGCTCGTATGCCGT1100.25900635742877326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.223687308688486No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC870.20485048269366612No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT830.19543206969625618No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC660.15540381445726395No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATATCGTATGCCGT650.15304921120791148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.15069460795855896No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG510.12008476571697668No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC500.11773016246762422No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG490.11537555921827172No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.11537555921827172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.11302095596891924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.11302095596891924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGGG259.911702E-6105.9306970
ATGCTTG150.001396412478.553654
TTATGCT150.00148832177.3049152
AGGGGGG2550.072.6975370
CGTATGG207.6688906E-570.2956641
ATCGTAT150.002322954669.1149939
TATCGTA150.002481867867.9733138
GAGTGTG150.003184679263.83651428
GGAGTGT150.003245450363.53325727
GGGGATC150.003338222263.0837318
TGTCGTA650.062.74460238
ATGTCGT650.062.4273137
TATGCTT200.00460259358.1467453
CATGTCG700.057.67654836
TATGGCG252.0406414E-457.67654843
TCATGTC700.057.1019435
CTCATGT700.056.9600734
CCGGCTT200.005570018755.41609247
GACGGCT200.00566209555.18741246
TCTTCTG2950.051.959650