FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822761
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCT28560.3471238913852261No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27950.3397098306798694No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATACATTGCCGAAG27700.33667128121046086No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25140.30555653464371796No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT23970.29133612312688617No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG23540.2861098180395036No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT22280.2707955287136848No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21270.2585197888572745No Hit
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGA20830.2531719417911155No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20560.24989030836415435No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC20480.24891797253394363No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20440.24843180461883826No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC20400.2479456367037329No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATA20330.24709484285229855No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20070.2439347514041137No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19410.2359129808048753No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATAC19240.2338467671656775No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC19180.2331175152930195No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC18910.22983588186605833No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18640.22655424843909716No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18550.2254603706301101No Hit
GACTATCACTGTCAATCAGCGGACACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT18410.22375878292724133No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA18010.21889710377618773No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17470.2123338369222654No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16280.197870341447881No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16270.19774879946910465No Hit
GGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGG16160.1964118377025649No Hit
GTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGA15820.19227941042416935No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA15760.1915501585515113No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15740.19130707459395863No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15440.18766081523066844No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA15080.1832853039947202No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14020.17040185424442822No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13950.16955106039299384No Hit
CCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGT13750.16712022081746702No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGG13610.16541863311459828No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT13120.15946307615455765No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC12970.15763994647291255No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12960.1575184044941362No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12730.1547229389822804No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAG12500.15192747347042457No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12190.14815967212835807No Hit
CCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGGGATCCCT12190.14815967212835807No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12170.14791658817080539No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12130.14743042025570002No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12050.1464580844254893No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12020.14609345848916028No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC11900.1446349547438442No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT11850.14402724484996252No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11630.14135332131688302No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11590.14086715340177766No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11450.13916556569890892No Hit
GATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGT11380.13831477184747454No Hit
GGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAGCCAGG11330.13770706195359284No Hit
GGATCACCTGCTCTGGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC11000.13369617665397365No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTC11000.13369617665397365No Hit
CCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA10760.13077916916334148No Hit
GACTATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAAT10760.13077916916334148No Hit
GCTCTGGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAG10750.13065762718456514No Hit
CACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGT10630.12919912343924905No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10550.12822678760903836No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10360.12591749001228789No Hit
AGCCAGGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCC10320.12543132209718255No Hit
CCACTGATAGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCG10270.12482361220330082No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10050.12214968867022137No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTCGGCAATGTATCTCCAGAGCAGGTGATCCTGGC9960.1210558108612343No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9850.11971884909469457No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGGGATCCCTGAGCGATT9800.11911113920081287No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCACATAGACTGTGTTGATGGACGTGTCCCTGGT9770.11874651326448385No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT9660.11740955149794413No Hit
GTGTTGATATATAAAGACATTCACAGGCCCTCAGGGATCCCTGAGCGATT9400.11424946004975928No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9370.11388483411343027No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACG9350.11364175015587759No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9340.11352020817710126No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCC9260.11254787234689052No Hit
CTGTCAATCAGCGGACACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCA9140.11108936860157446No Hit
CTATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCA9120.11084628464402178No Hit
GTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCGGACACCAG9070.11023857475014008No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCT9050.1099954907925874No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9010.10950932287748204No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGT8990.10926623891992936No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8920.10841544506849499No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC8910.10829390308971865No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8830.10732156725950792No Hit
CTGATAGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTC8820.1072000252807316No Hit
GCAGTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCC8790.10683539934440256No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8750.10634923142929721No Hit
ATATAAAGACATTCACAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT8680.10549843757786284No Hit
AGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC8640.10501226966275748No Hit
CCCTGAGGGCCTGTGAATGTCTTTATATATCAACACCACAGGGGCCTGGC8520.1035537659174414No Hit
GCTCAGGGACAACAGTCACGTTGACTATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAG8490.10318913998111237No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATACATTGCCGAAGCAGTA8430.10245988810845434No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG8340.10136601029946729No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8240.10015059051170389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCC208.588741E-568.876274
ACCGAGT200.006971963752.4606160
GTGGTAT2350.046.9231
TGGTATC2450.043.5747832
GTATATA503.8729286E-641.350891
CTACGCT1000.041.34586328
TCTCGTA350.001334938639.5043138
GTATCAA15400.039.158041
ATGCCGT459.748649E-538.50797744
TATATAG451.01195605E-438.264592
CTTATAC1100.037.591721
ACTACGC1100.037.5848627
GGTATCA6450.036.8631971
TACGCTG1151.8189894E-1235.9594829
GACTACG1201.8189894E-1234.44441626
TATACAC1550.033.327233
ATCAACG18850.032.5198333
CGAGACT1400.031.98215523
CCACGAG1305.456968E-1231.79291220
TCAACGC19350.031.501554
Produced by FastQC (version 0.11.5)