FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224726
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCT37190.3036597573661374No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATACATTGCCGAAG35720.2916570726840126No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33710.27524523852682153No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31260.2552407640566135No Hit
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGA30530.24928024717365355No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT29650.2420949665476196No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG29230.23866562806701255No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC28990.23670600607809422No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT27830.22723449979832225No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26770.21857950268059956No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26300.21474190961896783No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC26220.2140887022893284No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26190.21384374954071358No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26020.2124556839652298No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATA25440.2077199308253438No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC25280.20641351616606493No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24700.20167776302617893No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC24540.20037134836690002No Hit
GACTATCACTGTCAATCAGCGGACACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT24530.20028969745069508No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATAC24040.19628880255665349No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23880.1949823878973746No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA23830.19457413331634993No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22520.18387786329350403No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22170.18102008122633145No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21480.17538616800819123No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20880.1704871130358954No Hit
GTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGA20640.16852749104697703No Hit
GGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGG20560.1678742837173376No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA20440.1668944727228784No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC20270.16550640714739462No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20050.16371008699088613No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19650.16044405034268888No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18930.15456518437593386No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA18630.15211565688978596No Hit
CCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGT18590.15178905322496625No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17810.14542028176098162No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGG17300.1412560850345302No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCACATAGACTGTGTTGATGGACGTGTCCCTGGT16800.13717353922428363No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16780.1370102373918738No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT16370.1336625498274716No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAG16240.13260108791680753No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16240.13260108791680753No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16160.13194788058716805No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC16010.13072311684409413No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTC15760.12868184393897084No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15740.12851854210656097No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15700.12819193844174126No Hit
CCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGGGATCCCT15650.1277836838607166No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15580.1272121274472821No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATACTGCTTCGGCAATGTATCTCCAGAGCAGGTGATCCTGGC15340.12525250545836375No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15290.12484425087733911No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT15040.12280297797221582No Hit
GATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGT14990.12239472339119117No Hit
GGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAGCCAGG14970.12223142155878132No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14740.12035345048606791No Hit
GCTCTGGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAG14720.12019014865365804No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14650.11961859224022353No Hit
GGATCACCTGCTCTGGAGATACATTGCCGAAGCAGTATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC14550.11880208307817422No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14360.11725071567028054No Hit
CCGCTGGTGTCCGCTGATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATAGTCAACGTGA14200.11594430101100163No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14140.11545439551377205No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14050.11471953726792769No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCATCGG13950.11390302810587838No Hit
GACTATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAAT13790.11259661344659949No Hit
CACCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGT13770.11243331161418961No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13640.11137184970352552No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13630.11129019878732059No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT13370.10916727496599239No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGGTGTCCGCTGATTGACAGTGATAGT13340.10892232221737759No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13250.10818746397153321No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12970.10590123831779516No Hit
CCACTGATAGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCG12830.10475812549092614No Hit
AGCCAGGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCC12670.10345171083164723No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12600.10288015441821273No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12440.10157373975893383No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCACAGGCCCTCAGGGATCCCTGAGCGATT12280.10026732509965494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)