FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056517
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGCTGTCTGCTGATTGA41240.39033919946389883No Hit
CCCAAGCACCGCTGCTGTCTGCTGATTGACAGTGATAGTCAGCCTCGTCT36260.3432031855616142No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34600.3274911809275194No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33020.3125363813360315No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA28820.272783116599165No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC28810.2726884659688391No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC27230.25773366637735123No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT27040.2559353044011597No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT26950.2550834487282268No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCGAAG26470.2505402184725849No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC25370.24012864913673893No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25120.23776238337859212No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24560.23246194808034323No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24050.22763476593372373No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23530.22271293315677837No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC23450.22195572811417136No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22900.21674994344624837No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22630.21419437642744982No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG22450.2124906650815841No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA21590.20435071087355908No Hit
GACTATCACTGTCAATCAGCAGACAGCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT21340.20198444511541225No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC21230.20094328818182766No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21150.20018608313922068No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG20840.1972519135991186No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTCGAGTGGATGGGATGGATCAAACCTAACAGTGGTGA20220.1913835745189145No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGCTGTCTGCTGATTGACAGTGATAGT20210.19128892388858865No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC19870.18807080245750896No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19830.18769219993620548No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA19780.1872189467845761No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19660.18608313922066563No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19190.18163455959534963No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18870.1786057394249217No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC18680.17680737744873012No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17740.1679102181980981No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17290.16365093983343382No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT17210.16289373479082683No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16670.15778260075322972No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCATATAGACTGTGTTGATGGACGTGTCCCTGGT16530.1564574919286675No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16350.1547537805828018No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16080.15219821356400323No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGG15760.1491693933935753No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15620.1478442845690131No Hit
GTCTATATGGAGTTGAGCGGCCTGAGATCTGACGACACGGCCATGTATTACTGTGCGAGAGATGAGTCTGAGGTG15570.14737103141738372No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACC15430.1460459225928215No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15200.14386895809532643No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA15020.14216524674946074No Hit
GGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCAGAGGCCCTCAGG14970.14169199359783138No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT14880.14084013792489852No Hit
GTGTATATAGTTGCCGATGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC14790.13998828225196566No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT14770.1397989809913139No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAG14660.13875782405772932No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14580.13800061901512234No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCG14550.1377166671241447No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT14480.1370541127118636No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14000.1325108824562217No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTC13940.13194297867426646No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCA13930.13184832804394062No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13810.13071252048003015No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13750.1301446166980749No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13710.12976601417677142No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13680.1294820622857938No Hit
GCTCTGGAGATGCATTGCCGAAGCAATATGCTTATTGGTACCAGCAGAAG13670.12938741165546794No Hit
ACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGC13640.1291034597644903No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTCGGCAATGCATCTCCAGAGCAGGTGATCCTGGC13590.12863020661286093No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13500.12777835093992806No Hit
ATGTATTACTGTGCGAGAGATGAGTCTGAGGTGCCTACGATTTTGTCCTACTTTGACTACTGGGGCCAGGGAACC13370.12654789274569175No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13290.12579068770308474No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13250.12541208518178126No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13130.1242762776178708No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT12950.12257256627200509No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCATCGG12740.12058490303516177No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12590.11916514358027368No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12290.1163256246704975No Hit
CCGCTGCTGTCTGCTGATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA12040.11395935891235069No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCAGAC11890.1125395994574626No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCT11870.11235029819681083No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAACTTCGGCACCCAGAC11620.10998403243866403No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11560.1094161286567088No Hit
AGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC11440.10828032109279832No Hit
GGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGC11430.10818567046247245No Hit
CCCCTGTGGTGTTGATATATAAAGACATTCAGAGGCCCTCAGGGATCCCT11360.10752311605019135No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT11060.10468359714041517No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11000.10411569335845992No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCG10920.10335848831585293No Hit
CTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCT10810.10231733138226834No Hit
GGATCACCTGCTCTGGAGATGCATTGCCGAAGCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC10660.10089757192738025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGT200.00710391252.21351654
TATTCGC200.00724084651.96202546
GAACCGT200.00745340551.5831876
GTGGTAT2350.049.7869951
TGGTATC2700.043.308512
GTATCAA18900.041.694481
GGTATCA6900.040.8949471
GCTTGTA702.238994E-839.3275451
TCGCCCA459.446489E-538.7156456
ATCAACG22250.035.0809753
TCAACGC22250.035.0776564
CAACGCA22700.034.538655
AACGCAG23250.034.019026
CCTTTGT8050.032.0488670
CGCAGAG24500.031.5830428
AGAGTAC24750.031.12359411
CAGAGTA25100.030.9650810
AACAACC450.00443972930.92059953
ATACCTT450.004615096430.67673937
GCAGAGT25250.030.6449329
Produced by FastQC (version 0.11.5)