FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533732
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT41300.7737965870511793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.3190739921908374No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC15760.29527927873914245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.22314569859030373No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.15963067606963793No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC7250.1358359626179431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.12421964581475348No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT6550.12272076622724515TruSeq Adapter, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.11241596906312532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.1052962910224607No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT5540.10379741143495237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTC150.002124766670.8113642
AGGGGGG11050.070.2379570
TTGTTTC451.2834789E-859.8211861
CAGTCCG502.4006053E-455.93725270
AAGTACG1800.055.705481
GCTAAGT200.00684173252.7082441
CTAGTAT200.007284741451.87864339
GACGTCT351.6861655E-551.82954446
ACTAAAT303.5229983E-451.76577463
TCGCGTA800.051.34333838
CTTATAC12450.049.8816261
TATTCTG304.4412992E-449.39231550
GCGTATG1200.049.3776640
TTATACA12700.048.6131132
GCTCTAT200.0095139748.49039511
GGAGCAC200.00951749448.4858473
TATGTAG200.00951749448.4858474
TCTCATT304.921871E-448.3742446
TATACAC13150.047.195353
TATCGTA1050.045.63852338
Produced by FastQC (version 0.11.5)