FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248569
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT43220.13304319532692704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10600.045.72735640
CTCGTAT10000.040.4504939
GTATCAA102450.039.371711
CTTATAC13950.038.716591
CGTATGC13200.038.0895341
TATACAC14900.036.461783
GGTATCA45700.035.3053131
TATCAAC119050.034.1829382
ACGAGAC14800.033.9424222
TCTCGTA11750.033.49679638
ATCAACG123950.032.7213483
TCAACGC124550.032.7008864
AGGGGGG15700.032.2506870
TATGCCG14950.031.8550143
CAACGCA128400.031.6937585
ATCTCCG16300.031.2338610
AACGCAG131800.030.9539386
GCCGTCT15500.030.60441246
TTATACA18200.030.4137972
TATCGTA2050.030.31481638
Produced by FastQC (version 0.11.5)