FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223322
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41980.34316394211826484No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33130.2708199476507412No Hit
GTGTGTACCTTGCATGCAGTAATAAATTCCAACATCGTCAGCCTCCACCC32410.26493433454151893No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA30680.2507925141540821No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28500.23297218557338134No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27480.2246342336686498No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27470.22455248904213282No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA26480.2164597710169522No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25860.2113916041728997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24420.19962037795445514No Hit
GGTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24100.19700454990591193No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23600.19291731858006314No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTCATTTCACACTGAA21550.17615967014408307No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20550.1679852074923855No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20300.16594159182946108No Hit
CTCGTATACAGTGATGGAAACATCTACTTGAATTGGTTTCATCAGAGGCC19760.16152738199754438No Hit
CGTATACAGTGATGGAAACATCTACTTGAATTGGTTTCATCAGAGGCCAG19750.1614456373710274No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19230.15719491679214467No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18210.14885696488741312No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGATGAAA17150.14019203447661366No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16850.1377396956811044No Hit
AGGTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16700.13651352628334976No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16490.13479688912649326No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16230.13267152883705188No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15730.1285842975112031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15350.12547800170355802No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15190.12417008767928642No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14370.11746702830489437No Hit
GTGATGGAAACATCTACTTGAATTGGTTTCATCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT14230.11632260353365671No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14220.11624085890713973No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14200.11607736965410578No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13940.11395200936466443No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13640.11149967056915515No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13320.1088838425206119No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13120.10724894999027239No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT12960.10594103596600077No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12760.10430614343566126No Hit
GCAGTGGGTCAGGCACTCATTTCACACTGAAAATCAGCAGGGTGGAGGCT12400.10136333688105013No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12290.10046414598936339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.042.5755651
GTATCAA51250.039.4464341
TATCAAC59450.034.163672
CGTATGC2150.033.82819441
TCGTATG1900.032.7891940
CGAGACA2750.032.496823
ATCAACG63050.032.376533
TCAACGC63400.032.143594
CAACGCA64600.031.5996935
AACGCAG64900.031.4006716
ACTATCG450.00435619431.04125454
ATGCCGT2350.031.01181444
CGACTGG850.005324270629.79140570
GCCGTCT2000.029.52007946
ATCTCCG2800.029.44693610
CGCAGAG70000.029.005238
TCGCGGC605.255233E-428.90423243
CTTATAC3000.028.6512151
ATGAGTG17900.028.6471870
AGAGTAC71300.028.33183311
Produced by FastQC (version 0.11.5)