FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549697
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT25600.16519358300364523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12200.042.7787931
AGGGGGG11450.041.3214170
CTTATAC11550.041.0784151
TATACAC12400.039.8933683
GTATCAA91500.038.1860921
TTATACA12900.037.296512
GGTATCA40650.036.0989881
TAAGGCG13150.035.9676829
ACTAAGG13100.035.78838327
CGTATGC7950.035.76579741
CGAGACT13600.035.15265723
GGCGAAT12250.035.10041832
GAGACTA13550.035.04119524
GACTAAG13750.034.80964326
TGTCGTA1500.034.4444638
TCGTATG7950.034.39595440
AGACTAA14050.033.5679725
TACACAT14950.033.3154535
TATCAAC107800.033.0648462
ACGAGAC15050.032.4375422
Produced by FastQC (version 0.11.5)