FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549697
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103540.668130608757712No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC16760.10815017387269898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14000.048.089891
GTATCAA98350.036.704461
GGTATCA46100.035.8869631
TGGTATC20000.033.6487962
TATCAAC112500.032.0443272
TCAACGC117750.030.514664
ATCAACG120600.029.8776263
CAACGCA120600.029.8244575
AACGCAG123400.029.068366
GTATAGC704.4013534E-528.998931
ACGCAGA131600.027.2579887
CGCAGAG132650.027.0167398
AGAGTAC133050.026.93551411
CAGAGTA134050.026.8102410
GCAGAGT135850.026.455019
CCGTTAA1651.4733814E-1024.60276816
CGGGTAT1251.5269143E-724.36146217
AGTACGG89500.023.7947813
GAGTACG90900.023.61805512
GCCGTTA2053.6379788E-1223.0921615
Produced by FastQC (version 0.11.5)