FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580690
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45050.2850021193276354No Hit
GTATTACTGTCAATGTTTTGGTAGCTCACCTTTATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAA36190.22895064813467533No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA32570.20604925697005738No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30450.1926373925311098No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT29810.1885885277948238No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29140.18434987252402432No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28400.17966837267269356No Hit
GTGTATTACTGTCAATGTTTTGGTAGCTCACCTTTATTCACTTTCGGCCC28240.17865615648862207No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27400.17334202152224662No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26260.1661299812107371No Hit
AAGTAGTACTGTCCGCGGACGTAGTCACCGTCTAATAAAATTCTGGCACA24340.1539833870018789No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24220.15322422486382528No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAAGCAGCAGCTACTTAGCCTGGTACC23890.1511365289841778No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23730.1501243128001063No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23410.14809988043196326No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTAAGCAG22730.14379796164965933No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA22390.14164700225850735No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21970.13898993477531965No Hit
GTAATAGACGGCCGTGTCTGCGGCAGTCACAGAGTTCAGCCTCAGGGAGA21530.13620634026912298No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTG19790.12519848926734528No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19580.1238699555257514No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18480.11691096926025976No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC18060.11425390177707204No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17130.10837039520715636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16960.10729491551158038No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16770.10609290879299547No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC16680.10552353718945523No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16680.10552353718945523No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT16500.10438479398237478No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAATGTTTTGGTAGCTCA16460.10413173993635691No Hit
CCTTTATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC16270.10292973321777198No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15930.10077877382662001No Hit
GTCTATTACTGTGCCAGAATTTTATTAGACGGTGACTACGTCCGCGGACA15870.1003991927575932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15810.10001961168856638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6900.044.441111
GGTATCA19600.040.6069031
TGGTATC7600.040.3287052
GTATCAA45900.038.8080521
TAAGGCG2200.034.4685329
TCGTATG1154.5474735E-1133.1114340
TATCAAC55000.032.872632
ATCAACG57250.031.7611543
GGCGAAT2400.031.6221832
TCAACGC57750.031.5457974
GCGAATC2000.031.06101233
CAACGCA58800.030.9824815
AACGCAG60450.030.3646856
CGCAGAG65700.027.7259838
AGAGTAC65600.027.61164311
ATACGAC500.007782323827.56609226
CCCACGA2750.027.55039419
CGTATGC1653.6379788E-1227.28748941
ACGCAGA67600.027.0485787
TTAATCG2450.026.81049237
Produced by FastQC (version 0.11.5)