FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580690
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42480.2687433968709867No Hit
GTATTACTGTCAATGTTTTGGTAGCTCACCTTTATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAA35000.2214222902656435No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT28300.17903573755764887No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27820.17599908900543432No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA26980.1706849540390589No Hit
GTGTATTACTGTCAATGTTTTGGTAGCTCACCTTTATTCACTTTCGGCCC25870.16366270426206275No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25530.1615117448709108No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24650.1559445558585175No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23200.1467713466903694No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22580.1428490089770923No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22060.13955930637885985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21770.13772466454523025No Hit
AAGTAGTACTGTCCGCGGACGTAGTCACCGTCTAATAAAATTCTGGCACA21750.13759813752222133No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21140.13373906332044866No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20880.13209421202133245No Hit
GTAATAGACGGCCGTGTCTGCGGCAGTCACAGAGTTCAGCCTCAGGGAGA20480.12956367156115367No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAAGCAGCAGCTACTTAGCCTGGTACC20000.12652702300893914No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTG19990.12646375949743466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18640.11792318544433128No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18040.1141273747540631No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17590.11128051673636197No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTAAGCAG16990.1074847060460938No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT16970.10735817902308485No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16880.10678880741954463No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16570.10482763856290607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)