FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457907
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90850.038.6760031
GTGGTAT13450.037.574271
GGTATCA40900.037.4029461
TATCAAC104500.034.2594572
TCGTATG4850.034.23037340
TAAGGCG8800.031.86604529
ATCAACG116100.031.0716343
TCAACGC116000.031.0395774
CGTATGC5400.030.79856341
CTCGTAT4550.030.3660339
CAACGCA120200.030.0401785
CTTATAC10900.029.4477751
TGGTATC17600.029.280982
TATACAC11450.029.2106973
AACGCAG123800.029.1666376
TATGCCG6000.028.38765343
AGGGGGG12950.027.68331170
ACGCAGA134800.026.8069787
CGCAGAG136450.026.4833568
AGAGTAC138350.026.14538411
Produced by FastQC (version 0.11.5)