FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457907
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71340.2902469458771223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93750.039.5530661
GTGGTAT14000.038.499131
GGTATCA41650.037.83981
TATCAAC106100.034.8313672
TCAACGC114800.032.269634
ATCAACG114750.032.1662033
CAACGCA116550.031.6135185
AACGCAG120550.030.6801366
TGGTATC18050.029.6537112
ACGCAGA129500.028.6135797
CGCAGAG130950.028.3227758
AGAGTAC131300.028.16824311
CAGAGTA135700.027.33137310
GCAGAGT139450.026.6452879
AGTACGG82250.024.78133413
GAGTACG85900.024.04773712
TACGGGT17200.023.78534115
GAGTACT71950.023.07240712
AGTACTT73950.022.03175413
GACGATA1253.60776E-622.0072235
Produced by FastQC (version 0.11.5)