FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981443
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73750.039.400841
GGTATCA30800.037.715571
GTGGTAT11000.037.38181
TCGTATG3550.036.0826240
TAAGGCG6650.034.0425329
TATCAAC87050.033.7182042
ATCAACG88000.033.1596073
TCAACGC88800.033.0151444
TCTCGTA3300.032.44714438
CAACGCA91150.032.163965
CTCGTAT3550.032.1473539
GACTAAG6950.032.060926
CGTATGC4450.031.92703841
TGGTATC13100.031.6349012
ACGAGAC7150.031.62197322
TATGCCG4750.031.44029643
CTTATAC7850.031.4292851
AACGCAG96600.030.455696
GCGAATC5450.030.27612933
CGAATCT5100.028.33191134
Produced by FastQC (version 0.11.5)