FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981443
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50710.2559245963673949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76350.040.088781
GTGGTAT12800.037.6719361
TATCAAC87850.034.9409752
GGTATCA31800.034.5212671
ATCAACG92200.033.1316153
TCAACGC92250.032.930124
CAACGCA94650.032.2050555
TGGTATC15900.031.1724762
AACGCAG98600.030.8833546
CGCAGAG104700.029.2153438
ACGCAGA106050.028.7467547
AGAGTAC106750.028.52551511
CAGAGTA110350.027.68782610
CGTACCG500.00785508127.51390334
GCAGAGT111150.027.4892379
GGTTACG950.00867142926.96070170
AGTACGG73900.025.34118713
GAGTACG75050.025.00030712
ATACCGA1052.4543866E-522.7841286
GTACGGG82600.022.1733714
Produced by FastQC (version 0.11.5)