FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667945
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81151.2149203901518837No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC16160.24193608755211882No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC7710.11542866553383886No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG6810.10195450224195106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTT150.00283063465.872195
CGATACT355.7462027E-446.87957868
GTGGTAT12150.045.5788081
GTATCAA79650.034.1841051
CACATAC400.002884393633.76531634
TTATAGC400.003133446433.19958527
TATCGTG400.00325104932.95092
CGTTATA400.003257019732.93856411
ATTTGCT450.003851991431.82884448
GGTATCA43300.031.821331
TATCAAC89000.030.5443732
TGGTATC19400.028.53482
CTTATTC550.007350681327.88731665
AATGACG550.007350681327.88731665
ATCAACG98000.027.5923823
TCAACGC97500.027.5291374
AACGCAG98250.027.3230826
TGTACGA550.00861055826.99774458
CAACGCA99600.026.8845835
TATTAGT500.00969929626.3469054
Produced by FastQC (version 0.11.5)