FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336755
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61160.45752587422526936No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGACTCTTG49620.3711974146346937No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46690.3492786636294609No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41880.3132960041294029No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39170.29302302965016025No Hit
GTATAGAGTTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA37590.2812033618725944No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAA36570.27357294343391275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35950.2689348459515768No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35420.2649700206844186No Hit
CTCTTGGATAGTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAA34430.2575640263174628No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34340.256890754102285No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33580.251205344285228No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32890.24604359063553158No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG32780.24522070237253646No Hit
GTCTGGGACTCCAGAGGCCCGATAGGAAAGCGTATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA32400.242377997464008No Hit
ACTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACC31320.2342987308818744No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28290.21163189963755513No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27570.20624572191613272No Hit
GGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG27310.20430071329450797No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACC25880.1936031658755718No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25850.19337874180384587No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25730.19248104551694215No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25700.1922566214452162No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA25440.19031161282359146No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25140.18806737210633212No Hit
ATCCAAGAGTCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGT25010.18709486779551976No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25000.1870200597716111No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAGGAAACT24590.18395293079135666No Hit
CCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT23710.17736982468739595No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGA23640.17684616852003546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23010.17213326301379087No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22120.1654753488859215No Hit
GGTCTAGTCAGAGACTCTTGGATAGTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGT21910.16390438038383998No Hit
GCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCC21410.16016397918840775No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA21410.16016397918840775No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20980.15694723416033604No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20880.1561991539212496No Hit
CTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCC20640.15440376134744213No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCC20490.15328164098881245No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20390.15253356074972602No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20340.1521595206301828No Hit
GTTTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA20330.15208471260627415No Hit
GGCCAAAAGTGTAAGGAAACTCTATACGTTGCATGCAGTAATAAACTCCA20140.1506633601520099No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20100.15036412805637533No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20090.15028932003246667No Hit
TTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGA19940.14916719967383701No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19820.1482695033869333No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19780.1479702712912987No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTTAGGGAGA19740.14767103919566413No Hit
GTGATGATGGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACG19290.14430467811977513No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19210.14370621392850597No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19080.14273370961769358No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACTCTCC18980.14198562937860715No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18860.14108793309170342No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18720.14004062075698237No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC18580.13899330842226137No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18480.13824522818317492No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCA18430.13787118806363172No Hit
CCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18320.13704829980063662No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17970.13443001896383408No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17910.1339811708203822No Hit
GTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT17670.1321857782465747No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17480.13076442579231048No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17470.13068961776840185No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTCCACT17170.1284453770511425No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16110.1205157265168262No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15900.11894475801474465No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15860.11864552591911008No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15700.11744859753657177No Hit
GTGTAAGGAAACTCTATACGTTGCATGCAGTAATAAACTCCAACATCCTC15590.11662570927357667No Hit
GTTCAGTGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGG15480.11580282101058159No Hit
GCCCTGGCTTCTGCAGGTACCAGTCCAAATAGGTGTATCCATCATCACTA15370.1149799327475865No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15100.11296011610205312No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGA15090.11288530807814447No Hit
GTCCAAATAGGTGTATCCATCATCACTATCCAAGAGTCTCTGACTAGACC15040.11251126795860124No Hit
GGATACACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTAT14920.11161357167169751No Hit
GTCTCTGGTGACTCCATCAGCAGTGATAGTTACTACTGGAGCTGGATCCG14900.11146395562388021No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14770.11049145131306784No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14700.10996779514570734No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14690.10989298712179868No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14650.10959375502616411No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14610.10929452293052953No Hit
CACCTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGC14520.10862125071535174No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGTCTGGGTCATCACAATATCC14400.10772355442844798No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14340.10727470628499614No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14330.10719989826108749No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14230.10645181802200103No Hit
GTGTATTACTGTGCGAGAGGAGGGTCCCTCCGGACTACGGCGACAACCAT14190.10615258592636646No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14170.10600296987854918No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG14080.10532969766337139No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13950.104357193352559No Hit
TATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCT13940.10428238532865035No Hit
GGATATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT13890.10390834520910713No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGAGGATATTGTGATGACCCAGACTC13830.10345949706565526No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13820.10338468904174662No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAATAGGTGTATCCATCATCACTATCCAAGAGTCTCTGA13710.10256180077875153No Hit
GTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCC13680.1023373767070256No Hit
GGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAACGTATAGAGTTTCCTTACACTTTTGGCCAGGGGA13640.10203814461139102No Hit
TCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGATTTCACA13550.10136487239621322No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13470.10076640820494408No Hit
CTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCA13410.1003175600614922No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATACGCTTTCCTATCGGGCCTCTGGAGTCCCAGACAGGTT13400.10024275203758355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)