FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980800
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134081.367047308319739No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC10400.10603588907014683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9600.047.4209441
GTATCAA75050.036.754481
GGTATCA36900.036.6459241
TGGTATC13350.033.823682
CTTATCG450.003356711732.73768265
TACTAGC450.003487050332.48408563
TATCAAC84950.032.2496152
ATCAACG91300.030.1035253
TCAACGC92550.029.6939144
CAACGCA93850.029.2855835
AACGCAG95100.028.9360686
ACGCAGA102850.026.7229167
CGCAGAG104600.026.4046338
AATCTCG659.5669925E-426.0875130
AGAGTAC106950.025.85594211
CAGAGTA108450.025.6536110
GCAGAGT108850.025.4665079
ACGGGAT5950.024.91914216
ACGGGTA2450.023.38192216
AGTACGG72100.023.12308513
Produced by FastQC (version 0.11.5)